UCLA scientists isolate new population of pluripotent stem cells in fat removed during liposuction

Cell population could revolutionize regenerative medicine

2013-06-06

(Press-News.org) Researchers from the UCLA Department of Obstetrics and Gynecology have isolated a new population of primitive, stress-resistant human pluripotent stem cells easily derived from fat tissue that are able to differentiate into virtually every cell type in the human body without genetic modification.

The cells, called Multi-lineage Stress-Enduring (Muse-AT) stem cells from fat, or adipose, tissue, were discovered by "scientific accident" when a piece of equipment failed in the lab, killing all the stem cells in the experiment except for the Muse-AT cells. The research team further discovered that not only are Muse-AT cells able to survive severe stress, they may even be activated by it, said study senior author Gregorio Chazenbalk, an associate researcher with UCLA Obstetrics and Gynecology.

These pluripotent cells, isolated from fat tissue removed during liposuction, expressed many embryonic stem cell markers and were able to differentiate into muscle, bone, fat, cardiac, neuronal and liver cells. An examination of their genetic characteristics confirmed their specialized functions, as well as their capacity to regenerate tissue when transplanted back into the body following their "awakening."

"This population of cells lies dormant in the fat tissue until it is subjected to very harsh conditions. These cells can survive in conditions in which usually only cancer cells can live," Chazenbalk said. "Upon further investigation and clinical trials, these cells could prove a revolutionary treatment option for numerous diseases, including heart disease, stroke and for tissue damage and neural regeneration."

The results of the two-year study are published June 5, 2013 in the peer-reviewed journal PLOS ONE.

Purifying and isolating Muse-AT cells does not require the use of a cell sorter or other specialized, high-tech devices. They are able to grow either in suspension, forming cell spheres, or as adherent cells, forming cell aggregates very similar to human embryonic stem cell-derived embryoid bodies.

"We have been able to isolate these cells using a simple and efficient method that takes about six hours from the time the fat tissue is harvested," Chazenbalk said. "This research offers a new and exciting source of fat stem cells with pluripotent characteristics, as well as a new method for quickly isolating them. These cells also appear to be more primitive than the average fat stem cells, making them potentially superior sources for regenerative medicine."

Currently, embryonic stem cells and induced pluripotent stem cells - skin cells turned into embryonic-like cells - are the two main sources of pluripotent cells. However, both types can exhibit an uncontrolled capacity for differentiation and proliferation, leading to the formation of unwanted teratoma, or tumors. Little progress has been made in resolving that defect, Chazenbalk said.

Muse cells originally were discovered by a research group at Tokohu University in Japan and were derived from bone marrow and skin, rather than fat. That research group showed that Muse cells did not produce teratomas in animal models. Further research on the Muse-AT cells isolated at UCLA will need to be done to determine whether that cell population avoids production of teratomas.

In addition to providing a potential source of cells for regenerative medicine, Chazenbalk said the Muse-AT cells may provide a better understanding of cancer cells, the only other cells known to display such stress resistance.

Going forward, Chazenbalk and his team will use Muse-AT cells in animal models to regenerate damaged or dysfunctional tissue to determine how efficiently they grow and perform in the body and to gauge their potential for future clinical use.

"Because lipoaspiration is a safe and non-invasive procedure and Muse-AT cell isolation requires a simple yet highly efficient purification technique, Muse-AT cells could provide an ideal source of pluripotent-like stem cells," the study states. "Muse-AT cells have the potential to make a critical impact on the field of regenerative medicine."

INFORMATION:

The study was funded by the UCLA Department of Obstetrics and Gynecology, the Eunice Kennedy Shriver National Institute of Child Health & Human Development, the National Institutes of Health through the cooperative agreement U54 HD071836 and by the Department of Stem Cell Biology at Tohoku University in Japan.

ELSE PRESS RELEASES FROM THIS DATE:

Irish chronicles reveal links between cold weather and volcanic eruptions

2013-06-06

Medieval chronicles have given an international group of researchers a glimpse into the past to assess how historical volcanic eruptions affected the weather in Ireland up to 1500 years ago. By critically assessing over 40,000 written entries in the Irish Annals and comparing them with measurements taken from ice cores, the researchers successfully linked the climatic aftermath of volcanic eruptions to extreme cold weather events in Ireland over a 1200-year period from 431 to 1649. Their study, which has been published today, 6 June, in IOP Publishing's journal Environmental ...

First observation of spin Hall effect in a quantum gas is step toward 'atomtronics'

2013-06-06

Researchers at the National Institute of Standards and Technology (NIST) have reported* the first observation of the "spin Hall effect" in a Bose-Einstein condensate (BEC), a cloud of ultracold atoms acting as a single quantum object. As one consequence, they made the atoms, which spin like a child's top, skew to one side or the other, by an amount dependent on the spin direction. Besides offering new insight into the quantum mechanical world, they say the phenomenon is a step toward applications in "atomtronics"—the use of ultracold atoms as circuit components. The spin ...

Genetic mutation inherited from father's side linked to early puberty

2013-06-06

Boston, MA – Reaching puberty at an unusually early age can have adverse effects on social behavior and psychological development, as well as physical effects, including short stature, and lifelong health risks, such as diabetes, breast cancer and heart disease. Researchers at Brigham and Women's Hospital (BWH), in a multi-institutional collaboration with Boston Children's Hospital, the Broad Institute, and the University of Sao Paulo, Brazil, have identified that a genetic mutation leads to a type of premature puberty, known as central precocious puberty. Central precocious ...

Drug prevents post-traumatic stress-like symptoms in mice

2013-06-06

When injected into mice immediately following a traumatic event, a new drug prevents the animals from developing memory problems and increased anxiety that are indicative of post-traumatic stress disorder (PTSD). Howard Hughes Medical Institute scientists utilized mouse studies to suggest that a receptor called Oprl1 is altered in mice with PTSD-like symptoms. They then worked with a group at the Scripps Research Institute who had previously developed the Oprl1-targeted drug to examine its effects on fear memory modulation. The group has also shown that in humans, ...

University of Maryland School of Medicine finds gut bacteria play key role in vaccination

2013-06-06

Baltimore, Md. — June 5, 2013. The bacteria that live in the human gut may play an important role in immune response to vaccines and infection by wild-type enteric organisms, according to two recent studies resulting from a collaborative effort between the University of Maryland School of Medicine Institute for Genome Sciences and the Center for Vaccine Development. The first study, published online in PLOS ONE, http://umm.gd/13E3OHl, examines the impact of an oral typhoid vaccination on the microbiota, or populations of bacteria, in the human gut. The second study, also ...

Noble way to low-cost fuel cells, halogenated graphene may replace expensive platinum

2013-06-06

Ulsan, South Korea– The research team of Ulsan National Institute of Science and Technology (UNIST), Case Western Reserve University and University of North Texas have paved a new way for affordable commercialization of fuel cells with efficient metal-free electrocatalysts using edge-halogenated graphene nanoplatelets. Fuel cell technology has come a long way since the early days in the Apollo space program. Certainly the idea of running a car on pure hydrogen is an exciting prospect as the only emissions will be pure water. But how much will you be willing to pay ...

Ancient trapped water explains Earth's first ice age

2013-06-06

Tiny bubbles of water found in quartz grains in Australia may hold the key to understanding what caused the Earth's first ice age, say scientists. The Anglo-French study, published in the journal Nature, analysed the amount of ancient atmospheric argon gas (Ar) isotopes dissolved in the bubbles and found levels were very different to those in the air we breathe today. The researchers say their findings help explain why Earth didn't suffer its first ice age until 2.5 billion years ago, despite the Sun's rays being weaker during the early years of our planet's formation. "The ...

New study rebuts increase in willingness to cooperate from intuitive thinking

2013-06-06

The first study drew the conclusion that people cooperate more if they are forced to make decisions when pressed for time. The research group (Rand et al.) let the test subjects make decisions under pressure on social dilemmas. They found that the decisions made at the time were more oriented towards cooperation. The conclusion was that decisions under pressure that build on intuition promote cooperation. It has gotten a lot of attention and aroused a great deal of discussion. But it is not true, argues a group of researchers at several universities, including Linköping ...

Firefighting robot paints 3-D thermal imaging picture for rescuers

2013-06-06

Engineers in the Coordinated Robotics Lab at the University of California, San Diego, have developed new image processing techniques for rapid exploration and characterization of structural fires by small Segway-like robotic vehicles. A sophisticated on-board software system takes the thermal data recorded by the robot's small infrared camera and maps it onto a 3D scene constructed from the images taken by a pair of stereo RGB cameras. This allows small mobile robotic vehicles to create a virtual reality picture that includes a 3D map and temperature data that can ...

Bacillus thuringiensis Cry4B toxin kills Anopheles gambiae, a principal vector of malaria

2013-06-06

Researchers at The University of Texas at Dallas (UTD), led by Dr. Lee Bulla, have characterized a protein produced by the soil bacterium Bacillus thuringiensis subsp. israelensis (Bti), which is highly toxic against Anopheles gambiae, the principal mosquito vector of malaria. The protein toxin, which has been known for a number of years, is produced in a complex of other protein toxins, called Cry toxins, which also have mosquitocidal activity against other species of mosquito. The novelty of the research done by the UTD scientists is that they differentially fractionated ...

Study on new telerehabilitation stroke therapy model led by UTHealth Houston for underserved community in the Texas Rio Grande Valley

The capsid of the virus-derived retrotransposon Copia, a parasitic genome element, mediates synaptic plasticity at the Drosophila neuromuscular junction

UCLA scientists isolate new population of pluripotent stem cells in fat removed during liposuction

ELSE PRESS RELEASES FROM THIS DATE:

LAST 30 PRESS RELEASES: