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Pain-free microneedle influenza vaccine is effective, long-lasting

2013-09-04
(Press-News.org) Scientists have developed an influenza vaccine delivered via microneedle patch that provided 100 percent protection against a lethal influenza virus in mice more than one year after vaccination. They report their findings in the September 2013 issue of the journal Clinical and Vaccine Immunology.

Microneedles are a medium for delivery of influenza vaccine that avoids the pain associated with ordinary hypodermic needles. They are a mere seven tenths of a millimeter in length, and the volume of vaccine—a major contributor to pain—is minuscule.

Instead of a liquid containing whole killed or attenuated virus, this vaccine uses dry virus-like particles (VLPs) which simply coat the needles in the presence of a simple stabilizing agent, reducing the need for refrigeration — a potential boon for use in developing countries. The lower dose required when using microneedles also reduces the potential for side effects, such as lung inflammation.

"This method can induce higher levels of IgG2a antibodies as well as rapid recall immune responses following lethal challenge infection. Our previous study showed that microneedle vaccination induced higher levels of antibody-secreting cells in spleen and bone marrow compared to intramuscular vaccination," says Sang-Moo Kang of Georgia State University, a researcher on the study.

Earlier studies by this group showed that influenza VLP-coated microneedles actually produced higher short-term protection than conventional intramuscular immunization. In this study the researchers tested how effective the long-term protection of the vaccine was. Mice that received the vaccine were 100 percent protected from a lethal challenge with the influenza virus 14 months after vaccination.

Kang says his aim was to develop an easier and pain-free method of vaccine delivery. He also says that patients could probably use this system to vaccinate themselves.

### A copy of the study can be found online at http://bit.ly/asmtip0913a.

Clinical and Vaccine Immunology is a publication of the American Society for Microbiology (ASM). The ASM is the largest single life science society, composed of over 39,000 scientists and health professionals. Its mission is to advance the microbiological sciences as a vehicle for understanding life processes and to apply and communicate this knowledge for the improvement of health and environmental and economic well-being worldwide.


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[Press-News.org] Pain-free microneedle influenza vaccine is effective, long-lasting