Targeted immune booster removes toxic proteins in mouse model of Alzheimer's disease

(Press-News.org) Alzheimer's disease experts at NYU Langone Medical Center and elsewhere are reporting success in specifically harnessing a mouse's immune system to attack and remove the buildup of toxic proteins in the brain that are markers of the deadly neurodegenerative disease.

Reporting on their experiments in the journal Acta Neuropathologica Communications online Sept. 3, the researchers say the work advances the development of more effective clinical treatments for Alzheimer's because their immune booster reduced both amyloid beta plaques and tau tangles. Previous immunomodulatory efforts, they say, were singularly successful in decreasing amyloid beta deposits but made limited progress in reducing the buildup of tau proteins that are also a key disease characteristic linked to progressive brain damage.

"Our study results confirm that precisely boosting the immune system in mice can work effectively against Alzheimer's disease, a treatment model that could very well be applied in humans," says senior study investigator and neurologist Thomas Wisniewski, MD, a professor at NYU Langone.

If further animal testing proves successful, human clinical trials could begin within a year, says Wisniewski, who also serves as director of NYU Langone's Center for Cognitive Neurology and co-director of its Alzheimer's Disease Center.

In their written report, the research team calls their findings the first positive results for targeted stimulation of the natural, or innate immune system to both prevent the onset of Alzheimer's disease in animals bred to develop dementia, and to reverse its symptoms after the disease has already set in.

Using the dementia-prone mice, the team gave monthly injections of an immune system booster known as a type B, CpG, oligodeoxynucleotide that specifically binds to Toll-like receptor 9, or TLR9 for short. Activation of TLR9 triggers an immune response. Tests in mice that received the immune system booster injections showed that amyloid plaque formation was 50 percent to 70 percent less than in mice that received no therapy. Reductions in amyloid beta were almost the same for mice treated early on, at age 7 months, and before disease onset, compared to mice treated at age 11 months, which already had mild dementia. Immunostaining tests on brain tissue in treated mice showed one to two times fewer damaged neurons containing disease-related tau aggregates than in untreated mice.

Further cognitive, behavioral testing showed that treated mice made roughly half the number of mistakes in finding their way in water-reward mazes than untreated mice. Dementia-associated brain inflammation was also halved in treated mice, the researchers report, with "classic signs of a traditional immune response against both amyloid beta and tau proteins," as demonstrated by the presence of immune system cytokines and T-helper cells.

According to researchers, treated mice behaved "almost like normal" mice that never develop Alzheimer's-like symptoms.

Wisniewski says that unlike vaccines, which try to trigger an antibody-mediated stimulation of the body's immune system, his team's new approach attempts to "jump start and rejuvenate" the brain's natural microglial cell repair function. The breakdown of microglial repair — possibly from aging — has been linked for decades to the formation and removal of amyloid plaques and tau tangles in Alzheimer's disease.

Researchers say they selected TLR9 as the immune booster because it was a known stimulant for removing germs. A bacterial cytosine-guanosine sequence, or CpG, such as type B, CpG, oligodeoxynucleotide, was chosen to help activate TLR9 on brain cells because previous testing had shown it to be effective at triggering an immune response in both mice and humans, with very few side effects.

According to study lead investigator Henrieta Scholtzova, MD, PhD, a clinical fellow at NYU Langone, the latest series of experiments build on the team's original observations in 2009, also in mice, that CpG immune boosting was possible, and could reduce amyloid plaque formation.

"Now that we have shown that we can influence microglial function in Alzheimer's disease, to both prevent and repair tau-damaged brain tissue, then it is highly plausible that our treatment approach could also be applied to other neurodegenerative diseases tied to aging," says Scholtzova.

Alzheimer's disease remains the leading cause of dementia worldwide. The disease, which has no effective treatment, afflicts some 5.2 million Americans, mostly women, killing up to a half-million each year.

INFORMATION: Funding support for the study was provided by the U.S. National Institutes of Health. Corresponding federal grant numbers are RO1 AG20245, RO1 NS073502, ULI TR000038, and R01 CA016087. Additional research support was received from the Seix Dow Foundation and the Alzheimer's Association, and grant IIRG-12-239474.

Besides Wisniewski and Scholtzova, other researchers involved in these experiments were Peter Chianchiano, BA; Jason Pan, BA; Yanjie Sun; and Fernando Goni, PhD. Additional research support was provided by Pankaj Mehta, MD, PhD, at the New York State Institute for Basic Research in Developmental Disabilities on Staten Island, NY.

For more information, go to: http://adc.med.nyu.edu/researchers/affiliated-labs/dr-thomas-wisniewski http://www.actaneurocomms.org/content/2/1/101

About NYU Langone Medical Center:

NYU Langone Medical Center, a world-class, patient-centered, integrated academic medical center, is one of the nation's premier centers for excellence in clinical care, biomedical research, and medical education. Located in the heart of Manhattan, NYU Langone is composed of four hospitals — Tisch Hospital, its flagship acute care facility; Rusk Rehabilitation; the Hospital for Joint Diseases, the Medical Center's dedicated inpatient orthopaedic hospital; and Hassenfeld Children's Hospital, a comprehensive pediatric hospital supporting a full array of children's health services across the Medical Center — plus the NYU School of Medicine, which since 1841 has trained thousands of physicians and scientists who have helped to shape the course of medical history. The Medical Center's tri-fold mission to serve, teach, and discover is achieved 365 days a year through the seamless integration of a culture devoted to excellence in patient care, education, and research. For more information, go to http://www.NYULMC.org, and interact with us on Facebook, Twitter, and YouTube.

Media Contact: David March
212 .404.3528│david.march@nyumc.org