Above the noise

(Press-News.org) Osaka, Japan - Scientists from the Institute of Scientific and Industrial Research at Osaka University used machine learning methods to enhance the signal-to-noise ratio in data collected when tiny spheres are passed through microscopic nanopores cut into silicon substrates. This work may lead to much more sensitive data collection when sequencing DNA or detecting small concentrations of pathogens.

Miniaturization has opened the possibility for a wide range of diagnostic tools, such as point-of-care detection of diseases, to be performed quickly and with very small samples. For example, unknown particles can be analyzed by passing them through nanopores and recording tiny changes in the electrical current. However, the intensity of these signals can be very low, and is often buried under random noise. New techniques for extracting the useful information are clearly needed.

Now, scientists from Osaka University have used deep learning to "denoise" nanopore data. Most machine learning methods need to be trained with many "clean" examples before they can interpret noisy datasets. However, using a technique called "Noise2Noise," which was originally developed for enhancing images, the team was able to improve resolution of noisy runs even though no clean data was available. Deep neural networks, which act like layered neurons in the brain, were utilized to reduce the interference in the data.

"The deep denoising enabled us to reveal faint features in the ionic current signals hidden by random fluctuations," first author Makusu Tsutsui says. "Our algorithm was designed to select features that best represented the input data, thus allowing the computer to detect and subtract the noise from the raw data."

The process was repeated many times until the underlying signal was recovered. Essentially, many noisy runs were utilized to produce one clean signal.

"Our method may expand the capability nanopore sensing for rapid and accurate detection of infection diseases," explains senior author Takashi Washio. "This research may lead to much more accurate diagnostic tests, even when the underlying signal is very weak."

INFORMATION:

The article, "Deep learning-enhanced nanopore sensing of single-nanoparticle translocation dynamics" was published in Small Methods at DOI: https://doi.org/10.1002/smtd.202100191

About Osaka University Osaka University was founded in 1931 as one of the seven imperial universities of Japan and is now one of Japan's leading comprehensive universities with a broad disciplinary spectrum. This strength is coupled with a singular drive for innovation that extends throughout the scientific process, from fundamental research to the creation of applied technology with positive economic impacts. Its commitment to innovation has been recognized in Japan and around the world, being named Japan's most innovative university in 2015 (Reuters 2015 Top 100) and one of the most innovative institutions in the world in 2017 (Innovative Universities and the Nature Index Innovation 2017). Now, Osaka University is leveraging its role as a Designated National University Corporation selected by the Ministry of Education, Culture, Sports, Science and Technology to contribute to innovation for human welfare, sustainable development of society, and social transformation. Website: https://resou.osaka-u.ac.jp/en