Less is more
Small labeling pair for single-molecule super-resolution imaging
Published 2015-09-10 · Original archive entry · Explore research explainers
This news release is available in German.
Protein labeling with synthetic fluorescent probes is a key technology in chemical biology and biomedical research. The target proximity achieved by small-molecule probes is essential to exploit the full potential of super-resolution fluorescence microscopy. Single-molecule localization techniques provide high spatial resolution by reporting on the position of the fluorophore and thus only indirectly on the target molecule itself. Large labels, such as antibodies, can misleadingly position a fluorophore tens of nanometers away from the target. Since single-molecule localization microscopy can achieve almost the ultimate spatial precision ( END
Protein labeling with synthetic fluorescent probes is a key technology in chemical biology and biomedical research. The target proximity achieved by small-molecule probes is essential to exploit the full potential of super-resolution fluorescence microscopy. Single-molecule localization techniques provide high spatial resolution by reporting on the position of the fluorophore and thus only indirectly on the target molecule itself. Large labels, such as antibodies, can misleadingly position a fluorophore tens of nanometers away from the target. Since single-molecule localization microscopy can achieve almost the ultimate spatial precision ( END