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“Overall, the data presented in this study affirm that humanizing 3E10 preserves its crucial biological properties essential for therapeutic efficacy.”
BUFFALO, NY- October 21, 2024 – A new research paper was published in Oncotarget's Volume 15 on October 1, 2024, entitled, “Next-generation cell-penetrating antibodies for tumor targeting and RAD51 inhibition.”
As highlighted in the abstract, monoclonal antibody therapies for cancer have shown extraordinary clinical success in recent years. However, these strategies are primarily limited to targeting specific cell surface antigens, despite many disease targets being located intracellularly.
In their paper, researchers Madison Rackear, Elias Quijano, Zaira Ianniello, Daniel A. Colón-Ríos, Adam Krysztofiak, Rashed Abdullah, Yanfeng Liu, Faye A. Rogers, Dale L. Ludwig, Rohini Dwivedi, Franziska Bleichert, and Peter M. Glazer from the Departments of Therapeutic Radiology and Genetics at Yale University School of Medicine, Gennao Bio, and the Department of Molecular Biophysics and Biochemistry at Yale University report on the humanization of the full-length, nucleic acid-binding monoclonal lupus-derived autoantibody 3E10, which exhibits a novel mechanism for cell penetration and tumor-specific targeting.
The authors compare humanized variants of 3E10 and demonstrate that cell uptake relies on the nucleoside transporter ENT2. They also find that faster cell uptake and superior in vivo tumor targeting are associated with higher affinity nucleic acid binding.
“We show that one human variant retains the ability of the parental 3E10 to bind RAD51, serving as a synthetically lethal inhibitor of homology-directed repair in vitro.”
Continue reading: DOI: https://doi.org/10.18632/oncotarget.28651
Correspondence to: Peter M. Glazer - peter.glazer@yale.edu
Keywords: cancer, 3E10, cell penetration, nucleic acid binding, nucleic acid delivery, RAD51
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About Oncotarget:
Oncotarget (a primarily oncology-focused, peer-reviewed, open access journal) aims to maximize research impact through insightful peer-review; eliminate borders between specialties by linking different fields of oncology, cancer research and biomedical sciences; and foster application of basic and clinical science.
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In a paper out this month in Cell, researchers at Emory University have discovered a family of enzymes that ...
Climate change is increasingly influencing fire behaviour worldwide and intensifying fire smoke, endangering public health from air pollution caused by fires. These are the results of two new climate change impact attribution studies, both published in Nature Climate Change, with involvement of the Potsdam Institute for Climate Impact Research PIK. The first study finds 15.8 percent higher global burned areas over the period 2003 to 2019 due to climate change, intensifying fire activity especially in Australia, South America, Western North America and Siberia. These increasing fire dynamics offset the decrease in burned area ...
About The Study: The current patchwork of strategies to help patients manage high prescription drug costs highlights the structural and policy challenges within the U.S. prescription drug market that impede affordable access for some patients. While these strategies provide tangible solutions for clinicians to help patients access medically appropriate but costly medications, they do not address the root causes of high drug prices.
Corresponding Author: To contact the corresponding author, Hussain S. Lalani, MD, MPH, MSc, email hlalani@bwh.harvard.edu.
To access the embargoed study: Visit our For The Media website at this ...
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Integrative oncology is a patient-centered ...
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Corresponding Author: To contact the corresponding author, Parvati Singh, PhD, email singh.1704@osu.edu.
To access the embargoed study: Visit our For The Media website at this link https://media.jamanetwork.com/
(doi:10.1001/jamapediatrics.2024.4276)
Editor’s ...
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How would you summarize your study for a lay audience?
Prescription drugs can be expensive for patients, and many clinicians do not know how to respond when costs are too high. We reviewed the benefits and limitations of seven strategies that clinicians can use to help their patients navigate high-prescription drugs. These include co-payment cards, patient assistance ...
