What happens when some cells are more like Dad than Mom

(Press-News.org) NEW YORK, NY--New work by Columbia researchers has turned a textbook principle of genetics on its head and revealed why some people who carry disease-causing genes experience no symptoms.

Every biology student learns that each cell in our body (except sperm and eggs) contains two copies of each gene, one from each parent, and each copy plays an equal part in the cell. 

The new study shows that some cells are often biased when it comes to some genes and inactivate one parent’s copy. The phenomenon was discovered about a decade ago, but the new study shows how it can influence disease outcomes. The Columbia researchers looked at certain immune cells of ordinary people to get an estimate of the phenomenon and found that these cells had inactivated the maternal or paternal copy of a gene for one out of every 20 genes utilized by the cell. 

“This is suggesting that there is more plasticity in our DNA than we thought before,” says study leader Dusan Bogunovic, professor of pediatric immunology at Columbia University Vagelos College of Physicians and Surgeons.

“So in some cells in your body every 20th gene can be a little bit more Mom, a little bit less Dad, or vice versa.  And to make thing even more complicated, this can be different in white blood cells than in the kidney cells, and it can perhaps change with time.”

The results were published Jan. 1 in the journal Nature.

Why it matters The new study explains a longstanding puzzle in medicine: why do some people who’ve inherited a disease-causing mutation experience fewer symptoms than others with the same mutation? “In many diseases, we’ll see that 90% of people who carry a mutation are sick, but 10% who carry the mutation don’t get sick at all,” says Bogunovic, a scientist who studies children with rare immunological disorders at Columbia University Irving Medical Center.

Enlisting an international team of collaborators, the researchers looked at several families with different genetic disorders affecting their immune systems. In each case, the disease-causing copy was more likely to be active in sick patients and suppressed in healthy relatives who had inherited the same genes.

“There was some speculation that this bias toward one copy or the other could explain wide differences in the severity of a genetic disease, but no experimental evidence existed until now,” Bogunovic says.

Though the current work looked only at immune cells, Bogunovic says the selective bias for the maternal or paternal copy of a gene affected more than just immune-related genes. “We don’t see a preference for immune genes or any other class of genes, so we think this phenomenon can explain the wide variability in disease severity we see with many other genetic conditions,” he says, adding “this could be just the tip of the iceberg.”

The phenomenon could help explain diseases with flares, like lupus, or those that emerge following environmental triggers. It could also play a role in cancer.

Changing the future of treatments for genetic diseases? The study’s findings point to an entirely new paradigm for diagnosing and perhaps even treating inherited diseases.

The investigators propose expanding the standard characterization of genetic diseases to include patients’ “transcriptotypes,” their gene activity patterns, in addition to their genotypes. 

“This changes the paradigm of testing beyond your DNA to your RNA, which as we’ve shown in our study, is not equal in all cell types and can change over time,” says Bogunovic.

If researchers can identify the mechanisms behind selective gene inactivation, they may also be able to treat genetic diseases in a new way, by switching a patient’s gene expression pattern to suppress the undesirable copy. While emphasizing that such strategies are still far from clinical use, Bogunovic is optimistic: “At least in cell culture in the lab we can do it, so manipulation in that way is something that could turn somebody’s genetic disease into non-disease, assuming we are successful.”

Additional information "Monoallelic expression can govern penetrance of inborn errors of immunity(link is external and opens in a new window)," was published Jan. 1 in Nature.

All authors: O’Jay Stewart (Columbia), Conor Gruber (Icahn School of Medicine at Mount Sinai), Haley E. Randolph (Columbia), Roosheel Patel (Icahn School of Medicine at Mount Sinai), Meredith Ramba (Columbia), Enrica Calzoni (Columbia), Lei Haley Huang (Columbia), Jay Levy (Columbia), Sofija Buta (Columbia), Angelica Lee (Columbia), Christos Sazeides (Columbia), Zoe Prue (Columbia), David P. Hoytema van Konijnenburg (Boston Children’s Hospital and Harvard Medical School), Ivan K. Chinn (Baylor College of Medicine and Texas Children’s Hospital), Luis A. Pedroza (Columbia), James R. Lupski (Baylor), Erica G. Schmitt (Washington University School of Medicine), Megan A. Cooper (Washington University School of Medicine), Anne Puel (INSERM, University of Paris Cité, and Necker Hospital for Sick Children), Xiao Peng (Johns Hopkins), Stéphanie Boisson-Dupuis (INSERM, University of Paris Cité, and Necker Hospital for Sick Children), Jacinta Bustamante (INSERM, University of Paris Cité, Necker Hospital for Sick Children, and Rockefeller University), Satoshi Okada (Hiroshima University), Marta Martin-Fernandez (Columbia and Instituto de Salud Carlos III), Jordan S. Orange (Columbia), Jean-Laurent Casanova (INSERM, University of Paris Cité, Necker Hospital for Sick Children, Rockefeller University, and Howard Hughes Medical Institute), Joshua D. Milner (Columbia) & Dusan Bogunovic (Columbia).

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Columbia University Irving Medical Center (CUIMC) is a clinical, research, and educational campus located in New York City. Founded in 1928, CUIMC was one of the first academic medical centers established in the United States of America. CUIMC is home to four professional colleges and schools that provide global leadership in scientific research, health and medical education, and patient care including the Vagelos College of Physicians and Surgeons, the Mailman School of Public Health, the College of Dental Medicine, the School of Nursing. For more information, please visit cuimc.columbia.edu. 

 

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