(Press-News.org) NEW BRUNSWICK, N.J. – An team of Rutgers University scientists led by Richard H. Ebright and Eddy Arnold has determined the three-dimensional structure of the transcription initiation complex, the key intermediate in the process by which cells read out genetic information in DNA.
In a paper to be published in Science and released online today at Science Express, the Rutgers scientists show how the "molecular machine" responsible for transcription initiation – a protein complex that consists of the enzyme RNA polymerase and the initiation factor sigma – recognizes a specific site on DNA preceding a gene, binds to DNA, unwinds the DNA helix, and pre-organizes the unwound DNA to enable subsequent reactions.
"Determining the structure of a functional, specific transcription initiation complex has been a goal of researchers for three decades," said Ebright, a professor in the Department of Chemistry and Chemical Biology at Rutgers, a laboratory director at the Waksman Institute of Microbiology at Rutgers, and an investigator of the Howard Hughes Medical Institute.
The structure determined by the Rutgers researchers is the structure of a transcription initiation complex from a bacterium. The structure provides a foundation for understanding bacterial transcription initiation and transcriptional regulation and provides a starting point for developing new antibacterial agents that function by inhibiting bacterial transcription. Because the transcription machineries in bacteria and higher organisms are structurally and mechanistically related, the structure also provides a framework for understanding transcription and transcriptional regulation in higher organisms, including humans.
The structure defines the interactions that RNA polymerase and sigma make with the DNA site for transcription initiation, known as the "promoter." In particular, the structure defines interactions with a segment of the promoter that RNA polymerase and sigma unwind to form single-stranded DNA (the "transcription bubble") and specific DNA sequences that RNA polymerase and sigma recognize and bind to within this segment of the promoter (the "-10 element," the "discriminator element," and a new DNA sequence identified in this work, the "core recognition element").
The structure shows that a first part of sigma recognizes the -10 element through contacts with single-stranded DNA that entail the unstacking and insertion of DNA bases of the -10 element into pockets. A second part of sigma recognizes the discriminator element through contacts with single-stranded DNA that entail the unstacking and insertion of a DNA base of the discriminator element into a pocket. A third part of sigma contacts the other strand of DNA and pre-organizes it to serve as the template for RNA synthesis. Finally, RNA polymerase recognizes the core recognition element through contacts with single-stranded DNA, unstacking and inserting a DNA base into a pocket.
"This study represents a very significant contribution to our understanding of the workings of this central macromolecular machine of gene expression," said Peter von Hippel, professor of biophysical chemistry and molecular biology at the University of Oregon, who was not part of the study. "A particular significance of this work is the very systematic way the researchers built nucleic acid scaffolds bound to various nucleic acid and protein complexes involved in the various steps of initiation and were able to show in detail how the sigma initiation factor interacts with the various individual nucleotide residues involved in the recognition of the important elements of the promoter."
"While the structures of several RNA polymerase enzymes are known, no previous structures show the polymerase, including an initiation factor and the promoter DNA, poised to begin elongation," said Peter Preusch of the National Institutes of Health's National Institute of General Medical Sciences, which partially funded the work. "This detailed three-dimensional structure informs our understanding of how transcription initiation occurs and may lead to new ways to manipulate this fundamental process for therapeutic purposes."
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The research was funded by the National Institute of General Medical Sciences and the National Institutes for Allergy and Infectious Diseases, both part of the National Institutes of Health. Data for the study were collected at beamline X25 of the National Synchrotron Light Source, which is supported by the Department of Energy and the National Center for Research Resources and the National Institute of General Medical Sciences of the National Institutes of Health. Additional data were collected at the Macromolecular Diffraction Facility of the Cornell High Energy Synchrotron Source, which is supported by the National Science Foundation and the National Institute of General Medical Sciences.
Rutgers researchers unveil 3-D structure of 'molecular machine' that initiates DNA transcription
Structure sought for three decades; starting point for developing new antibacterial agents
2012-10-19
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[Press-News.org] Rutgers researchers unveil 3-D structure of 'molecular machine' that initiates DNA transcriptionStructure sought for three decades; starting point for developing new antibacterial agents