PRESS-NEWS.org - Press Release Distribution
PRESS RELEASES DISTRIBUTION

Improving DNA amplification from problematic plants

A reagent added to standard PCR helps overcome compounds that block DNA amplification in plants

2013-01-04
(Press-News.org) The polymerase chain reaction (PCR) is a common technique used to amplify, or copy, pieces of DNA. Amplified DNA is then used in genetic analyses for everything from medicine to forensics. In plant research, PCR is a vital step in detecting and sequencing genes, and its applications are endless. However, compounds found in plants often inhibit PCR. Researchers at the University of Southern Mississippi discovered that the use of an additive allows PCR to successfully amplify DNA from once problematic plants.

PCR is widely used in plant sciences but is not 100 percent reliable. Many plant researchers encounter roadblocks when implementing PCR. For example, many plant species contain phenolic compounds that deter herbivores. These compounds are often extracted along with plant DNA and can stop PCR from working.

Graduate student Tharangamala Samarakoon and colleagues have found a technique to overcome many of these inhibitory plant compounds. They added a reagent to the PCR mixture that contains three ingredients: trehalose, bovine serum albumin, and polysorbate-20 (all three abbreviated TBT-PAR). "Unlike several other studies, TBT-PAR works at the PCR stage instead of at the DNA extraction stage, so it has promise for pigeon-holed and half-forgotten extractions that previously failed to be amplified using PCR," says Samarakoon. The authors published their research in the January issue of Applications in Plant Sciences.

Samarakoon tested the TBT-PAR reagent on DNA extracted from tropical and temperate species across four plant families, including Achariaceae, Asteraceae, Lacistemataceae, and Samydaceae. PCR with TBT-PAR successfully amplified DNA for all species, whereas standard DNA extraction and PCR techniques consistently failed.

TBT-PAR enhanced PCR for DNA extracted from fresh, silica-dried, and herbarium plant material. "Since we study tropical plants, many of which are geographically restricted or rare," explains Samarakoon, "herbarium material is sometimes all that we have available for DNA extraction, and curators are gracious to allow even a small destructive sampling for a single extraction attempt. We want that one attempt, of course, to be successful." Samarakoon predicts that inhibitory plant compounds could be the underlying cause of many PCR failures in herbarium specimens and hopes TBT-PAR will have widespread benefits in herbarium specimen DNA amplification.

TBT-PAR was first used in the PCR detection of a shrimp virus by co-author Shiao Wang and his colleagues. "The additive has also been helpful in a colleague's lab where they had trouble amplifying DNA from gopher tortoise ticks, so its utility extends beyond plants," comments Samarakoon. TBT-PAR has the potential for broad use in PCR techniques across DNA samples, species, and taxa.

The article will be published in the first issue of Applications in Plant Sciences (APPS), a new journal released by the Botanical Society of America. Theresa Culley, Editor-in-Chief of APPS, describes the new journal as a venue to "expedite the dissemination of innovative information encompassing all areas of the plant sciences, including but not limited to genetics, structure, development, evolution, systematics, and ecology." APPS publishes new methods in plant sciences—an important niche to fill in an age of rapid technological advances.

INFORMATION:

Culley, T. M. 2013. Changing technologies offer new opportunities in the plant sciences. Applications in Plant Sciences 1(1): 1200008. doi:10.3732/apps.1200008

Samarakoon, T., S. Y. Wang, and M. H. Alford. 2013. Enhancing PCR amplification of DNA from recalcitrant plant specimens using a trehalose-based additive. Applications in Plant Sciences 1(1): 1200236. doi:10.3732/apps.1200236

Applications in Plant Sciences (APPS) is a new, online-only, open access journal focusing on new tools, technologies, and protocols in all areas of the plant sciences. It is published by the Botanical Society of America, a non-profit membership society with a mission to promote botany, the field of basic science dealing with the study and inquiry into the form, function, development, diversity, reproduction, evolution, and uses of plants and their interactions within the biosphere. The first issue of APPS published 2 January 2013 and is available as part of BioOne's Open Access collection.

For further information, please contact the APPS staff at apps@botany.org.

END



ELSE PRESS RELEASES FROM THIS DATE:

Plvap/PV1 critical to formation of the diaphragms in endothelial cells

2013-01-04
PV1 expression in vascular endothelium is required for survival PV1 is required for the formation of stomatal and fenestral diaphragms Lack of diaphragms in fenestrated endothelia causes vascular leak of plasma proteins Vascular leak results in severe hypoproteinemia and hypertriglyceridemia Dartmouth scientists have demonstrated the importance of the gene Plvap and the structures it forms in mammalian physiology in a study published in December by the journal Developmental Cell. "The knowledge generated and the animal models created will allow a better understanding ...

Finding Chicago's food gardens with Google Earth

2013-01-04
Urban agriculture is promoted as a strategy for dealing with food insecurity, stimulating economic development, and combating diet-related health problems in cities. However, up to now, no one has known how much gardening is taking place in urban areas. Researchers at the University of Illinois have developed a methodology that they used to quantify the urban agriculture in Chicago. John Taylor, a doctoral candidate working with crop sciences researcher Sarah Taylor Lovell, was skeptical about the lists of urban gardens provided to him by local non-governmental organizations ...

Dopamine-receptor gene variant linked to human longevity

2013-01-04
Irvine, Calif., Jan. 3, 2013 — A variant of a gene associated with active personality traits in humans seems to also be involved with living a longer life, UC Irvine and other researchers have found. This derivative of a dopamine-receptor gene – called the DRD4 7R allele – appears in significantly higher rates in people more than 90 years old and is linked to lifespan increases in mouse studies. Robert Moyzis, professor of biological chemistry at UC Irvine, and Dr. Nora Volkow, a psychiatrist who conducts research at the Brookhaven National Laboratory and also directs ...

2 NASA satellites see Cyclone Dumile over La Reunion and Mauritius

2 NASA satellites see Cyclone Dumile over La Reunion and Mauritius
2013-01-04
NASA's Aqua and Terra satellites captured visible and infrared data on Tropical Cyclone Dumile as it slammed into the islands of La Reunion and Mauritius in the Southern Indian Ocean. The Moderate Resolution Imaging Spectroradiometer (MODIS) instrument that flies aboard NASA's Terra satellite captured a visible image of Cyclone Dumile on Jan. 3, 2013 at 0650 UTC (1:50 a.m. EST/U.S.) The image showed Dumile's center was about 85 nautical miles (97.8 miles/157.4 km) northwest of Reunion Island and Mauritius, and the strongest thunderstorms appeared to be southwest of the ...

NASA sees Tropical Depression Sonamu form near Philippines

NASA sees Tropical Depression Sonamu form near Philippines
2013-01-04
The first Tropical Depression of 2013 formed the western North Pacific Ocean today, and NASA's Terra satellite captured an infrared image of the "birth." Tropical Depression Sonamu, otherwise known as Tropical Depression 01W developed near 8.6 north latitude and 118.6 east longitude, about 185 nautical miles (213 miles/342.6 km) northwest of Zamboanga, Philippines. Sonamu's center is located in the Sulu Sea and is expected to cross the southern end of Palawan before moving into the open waters of the South China Sea. Sonamu developed from low pressure System 92W. At ...

New rat model for muscle regeneration after trauma-related soft tissue injury

New rat model for muscle regeneration after trauma-related soft tissue injury
2013-01-04
New Rochelle, NY, January 3, 2013—Penetrating soft tissue injuries that may be caused by bullet wounds or motor vehicle accidents, or exposure to explosive devices in military settings, can cause muscle loss resulting in functional disability and cosmetic deformity. Efforts underway to develop tissue engineering solutions to repair and replace damaged and lost muscle will benefit greatly from the availability of robust animal models to test these innovative therapeutic strategies. A new rat model that simulates traumatic or surgical muscle tissue loss in humans is described ...

Best evidence yet that dinosaurs used feathers for courtship

2013-01-04
(Edmonton) A University of Alberta researcher's examination of fossilized dinosaur tail bones has led to a breakthrough finding: some feathered dinosaurs used tail plumage to attract mates, much like modern-day peacocks and turkeys. U of A Paleontology researcher Scott Persons followed a chain of fossil evidence that started with a peculiar fusing together of vertebrae at the tip of the tail of four different species of dinosaurs, some separated in time and evolution by 45 million years. Persons says the final vertebrae in the tails of a group of dinosaurs called oviraptors ...

The Riviera Comedy Club in Las Vegas Begins 2013 with Comedian Don Barnhart

2013-01-04
The Riviera Comedy Club in Las Vegas Presents Comedian Don Barnhart Beginning Jan 7th - 13th, Comedian Don Barnhart brings his award-winning stand up comedy show to The Riviera Comedy Club in Las Vegas. Barnhart combines clever, well-written material with an improvisational flair that keeps audiences of all demographics howling with laughter and coming back for more. In 2012, Barnhart's show was named "Best Bet" by the Las Vegas Review/Journal. Appearing alongside Barnhart is comedian Kathleen Dunbar. Show times are 8:30pm Mon-Sun and tickets are $19.99 ...

Nonprofit for Women's Job Placement is Voted the Best Company to Work For

2013-01-04
A company that finds jobs is ironically the best company to work for. Every Year, Atlanta highlights the best companies in the metro area, and this time, Every Woman Works is one of them. Among the 45 companies chosen locally, Every Woman Works Inc., has been voted as one of the Best and Brightest Companies to Work For in 2012. Each year, the Best and Brightest Companies to Work For competition identifies and honors organizations that display a commitment to excellence in their human resource practices and employee enrichment. Organizations are assessed based on categories ...

Preferred Healthcare Funding, LLC Announces Launch Today

2013-01-04
Preferred Healthcare Funding, LLC announces its launch today to offer healthcare providers a solution for the immediate and ongoing resolution of their current and future self-pay and self-pay after insurance patient accounts receivable. Preferred Healthcare Funding, LLC (the "Company") will serve healthcare providers nationwide with a full complement of self-pay revenue cycle acquisition services. The Company has solutions in place for the purchase of self-pay and self-pay after insurance accounts at any stage in the revenue cycle from early out, primary to ...

LAST 30 PRESS RELEASES:

Healthy kidneys despite hypertension

Webb Telescope finds towering plume of water escaping from Saturn moon

Ghahari studying correlated and topological phases in Graphene Van der Waals (vdW) heterostructures

A telescope’s last view

An algorithm for sharper protein films

4,000-year-old plague DNA found – the oldest cases to date in Britain

The making of a Mona Lisa hologram

How insects track odors by navigating microscale winds

Sleep health before SARS-CoV-2 infection and risk of long COVID

Association between heart attack and cognition

Volunteering, health, and well-being of children and adolescents

Use of metabolic and bariatric surgery among youth

Racial, ethnic, and language disparities in identifying and mitigating central line–associated bloodstream infections

Philosophy aligns with economics on how to value future generations in climate policy

Researchers proposed a deep neural network-based 4-quadrant analog sun sensor calibration

Obesity increases risk of mental disorders throughout life

Researchers confirm the protective effect of hydrogen inhalation on declining brain function under hindlimb unloading conditions and disclose the underlying mechanism

Study finds 107-million-year-old pterosaur bones are oldest in Australia

Quarter-ton marsupial roamed long distances across Australia’s arid interior

Source-shifting metastructures composed of only one resin for location camouflaging

Code-switching in intercultural communication: Japanese vs Chinese point of view

Trials will investigate if rock dust can combat climate crisis

Women with a first normal weight offspring and a small second offspring have increased risk of cardiovascular mortality

ENDO 2023 press conferences to highlight emerging technology and diabetes research

New tool may help spot “invisible” brain damage in college athletes

The next generation of solar energy collectors could be rocks

Hidden in plain sight: Windshield washer fluid is an unexpected emission source

Humans evolved to walk with an extra spring in our step

Bile acid receptor could be innovative target in protecting the vision of premature newborns

Study finds similar quality and cost of care for patients treated by an allopathic (M.D.) or osteopathic (D.O.) physician

[Press-News.org] Improving DNA amplification from problematic plants
A reagent added to standard PCR helps overcome compounds that block DNA amplification in plants