Titanium oxide nanotubes facilitate low-cost laser-assisted photoporation
2021-01-25
(Press-News.org) Overview:
A research team at the Department of Mechanical Engineering at Toyohashi University of Technology developed a nanosecond pulse laser-assisted photoporation method using titanium-oxide nanotubes (TNTs) for highly efficient and low-cost intracellular delivery. The proof of concept for the possibility of intracellular delivery after irradiation with nanosecond pulse laser on TNTs was validated. TNTs were formed on titanium sheets using the electrochemical anodization technique at different voltages and times. HeLa - human cervical cancer cells were cultured in the nanotubes and submerged in a solution of biomolecules. After cells were exposed to nanosecond pulse laser, we successfully delivered propidium iodide (PI) and fluorescent dextran into cells with high efficiency and cell viability.
Details:
A research team at the Department of Mechanical Engineering at Toyohashi University of Technology developed a nanosecond pulse laser-assisted photoporation method using titanium-oxide nanotubes (TNT) for highly efficient and low-cost intracellular delivery. The results of their research will be published in the Applied Surface Science on 30 March 2021, 148815. (Available online 24 December 2020).
The potential to deliver external molecules into living cells with high cell viability and transfection ability is of great interest in cell biology for diagnostics, drug delivery, and therapeutic development towards cell therapy and regenerative medicine. Over many years, drug delivery systems have advanced to attain more control of drug dosage, targeted delivery, and reduced side effects. These techniques can be classified as viral, physical, or chemical methods.
Among these methods, photoporation is emerging and has become popular for intracellular delivery in the last few years, owing to less invasiveness. In this method, gold nanoparticles, which absorb pulsed light, are dispersed in a solution to perforate the cells, however, the materials are expensive. It is desirable to use nanomaterials that are less expensive while maintaining high delivery efficiency and cell viability.
The research group designed and fabricated a cost-effective nanotube array for photoporation based intracellular delivery. TNTs were formed on titanium sheets at different voltages and times using the electrochemical anodization technique. X-ray photoelectron spectroscopy (XPS) revealed the presence of different titanium oxide species such as TiO2 and TixOy (TiO/Ti2O3/Ti3O5). TNTs formed by different anodization voltages and times had different concentrations of such oxidation species along with a minor quantity of Ti metal (Ti0). Owing to the formation of oxygen defects, nanotubes have quasi-metallic and metallic properties. These properties of the nanotubes may facilitate the intracellular delivery by various mechanisms after irradiation with a nanosecond pulse laser.
HeLa - human cervical cancer cells were cultured on TNTs and a biomolecular solution was introduced. After exposure to a 532-nm pulse laser on nanotubes, we successfully delivered propidium iodide (PI) and dextran into the HeLa - human cervical cancer cells with high efficiency and cell viability.
Possible principles of cell membrane perforation include thermal-mediated nanobubbles, photochemical induced reactive oxygen species (ROS), heat transfer from nanotubes to the cell membrane, and localized surface plasmon resonance high electromagnetic field enhancement on each nanotube. This leads to the formation of cavitational nanobubbles in each cell membrane-nanotube interface that may rapidly grow, coalesce, and collapse to cause explosions, resulting in cell membrane perforation, which enables biomolecules to be delivered from the outside to inside the cells. "The precise mechanism for the intracellular delivery on TNT-based photoporation is still unclear. Intracellular delivery may happen by the combination of the mechanisms," says L. Mohan, a researcher, at Toyohashi University of Technology.
Moeto Nagai, the team leader, at Toyohashi University of Technology, believes that titanium oxide nanotubes could be a versatile and low-cost platform for intracellular delivery using pulsed laser. This device's prominent features have parallel and controlled uniform delivery with high efficiency and cell viability and it is potentially applicable for cellular therapy and regenerative medicine.
INFORMATION:
Funding agency:
This work was supported by JSPS KAKENHI Grant Numbers 16H06074 and 20H02115 and JSPS Postdoctoral Fellowship for Research in Japan and by the Leading Initiative for Excellent Young Researchers, MEXT.
Reference:
Mohan, L., Srabani Kar, Ren Hattori, Miho Ishii-Teshima, Parthasarathi Bera, Sounak Roy, Tuhin Subhra Santra, Takayuki Shibata, and Moeto Nagai. "Can titanium oxide nanotubes facilitate intracellular delivery by laser-assisted photoporation?" Applied Surface Science, Volume 543 (2021) 148815:
http://www.ncbi.nlm.nih.gov/pubmed/148815. doi.org/10.1016/j.apsusc.2020.148815
[Attachments] See images for this press release:
ELSE PRESS RELEASES FROM THIS DATE:
2021-01-25
There isn't much in Kamchatka, a remote peninsula in northeastern Russia just across the Bering Sea from Alaska, besides an impressive population of brown bears and the most explosive volcano in the world.
Kamchatka's Shiveluch volcano has had more than 40 violent eruptions over the last 10,000 years. The last gigantic blast occurred in 1964, creating a new crater and covering an area of nearly 100 square kilometers with pyroclastic flows. But Shiveluch is actually currently erupting, as it has been for over 20 years. So why would anyone risk venturing too close?
Researchers from Washington University in St. Louis, including Michael Krawczynski, assistant professor of earth and planetary sciences in Arts & Sciences and graduate student ...
2021-01-25
Abusive bosses may retain their positions by taking superficial steps to repair their social images following outbursts, without acting meaningfully to change their behaviors, according to research led by a University of Wyoming business management expert.
Shawn McClean, an assistant professor in UW's College of Business, joined colleagues from the University of Iowa, the University of Nebraska-Lincoln and Texas A&M University in conducting the research, which appears in the journal Personnel Psychology. Their study also was featured in Harvard Business Review, a preeminent business magazine.
"Our study shows that supervisors are often driven by simply repairing their social image rather than ...
2021-01-25
Male breast cancer patients were found to have a high prevalence of cardiovascular conditions, in a small study of this rare patient population presented at the American College of Cardiology's Advancing the Cardiovascular Care of the Oncology Patient Virtual course.
"Due to the rarity of male breast cancer, there is no cardiovascular data from larger clinical trials or population studies. The lack of large data makes it even more important to individualize cardiovascular assessment and management based on each patient's unique oncologic, therapeutic and pre-existing cardiovascular risk profile to support them through cancer treatment into survivorship," said Michael Ibrahim, fourth year ...
2021-01-25
PHILADELPHIA, PA - January 25, 2021 - Opertech Bio, Inc., today announced the publication of a seminal research article describing the application of its pioneering TāStation® technology to the pharmacological characterization of human taste discrimination. The findings are published in the peer-reviewed Journal of Pharmacology and Experimental Therapeutics, JPET.
The paper, entitled "Rapid throughput concentration-response analysis of human taste discrimination," is the first to quantitatively define the concentration-response function for human taste discrimination, a crucial step in understanding ...
2021-01-25
In a study of local rivers, experts at the University of Nottingham in the UK have discovered more invertebrates - animals without a backbone, such as insects and snails - living on litter than on rocks.
In urban rivers where there are no better alternatives, litter provided the largest, most stable and complex habitat available for invertebrates to live on.
The findings could have important implications for the management of urban rivers, including how river clean-up events are conducted.
The research team, in the School of Geography, studied three local rivers; the River Leen, Black Brook, and Saffron Brook, in ...
2021-01-25
DISCOVERY BRIEF:
The first new skull of a rare species of the dinosaur Parasaurolophus (recognized by the large hollow tube that grows on its head) discovered in 97 years.
Exquisite preservation of the new skull gives paleontologists their first opportunity to definitively identify how such a bizarre structure grew on this dinosaur.
For the first time, this study found characteristics to link tube-crested dinosaur species found in southern North America (New Mexico, Utah), distinct from the only northern species (Alberta).
The locality, in northwestern New Mexico, is dated to about 75 million years ago, a time when North America was divided by a shallow sea and teemed with duckbilled dinosaurs, horned dinosaurs and early tyrannosaurs.
Fossils from ...
2021-01-25
The cover for issue 2 of Oncotarget features Figure 4, "Combination therapy TA99/ICB reduced the lung tumor burden in the B16 model of metastases," published in "Improved therapeutic efficacy of unmodified anti-tumor antibodies by immune checkpoint blockade and kinase targeted therapy in mouse models of melanoma" by Pérez-Lorenzo, et al. which reported that here, the authors showed that removing immune suppression and enhancing stimulatory signals increased the anti-tumor activity of unmodified TA99 antibodies with a significant reduction of ...
2021-01-25
The cover for issue 1 of Oncotarget features Figure 2, "Results in clinical trials," published in "Drug resistant cells with very large proliferative potential grow exponentially in metastatic prostate cancer" by Blagoev, et al. which reported that most metastatic cancers develop drug resistance during treatment and continue to grow, driven by a subpopulation of cancer cells unresponsive to the therapy being administered.
There is evidence that metastases are formed by phenotypically plastic cancer cells with stem-cell-like properties.
Currently, the population structure and growth dynamics ...
2021-01-25
The cover for issue 52 of Oncotarget features Figure 1, "Volcano plots of DNA methylation in tumor tissues compared with nontumor tissue," published in "Reduction of T-Box 15 gene expression in tumor tissue is a prognostic biomarker for patients with hepatocellular carcinoma" by Morine, et al. which reported that the authors conducted a genome-wide analysis of DNA methylation of the tumor and non-tumor tissue of 15 patients with Hepatocellular carcinoma (HCC), and revealed TBX15 was the most hypermethylated gene of the tumor.
Another validation set, which comprised 58 HCC with radical resection, was analyzed to investigate the relationships between tumor phenotype and TBX15 mRNA expression.
TBX15 mRNA levels in tumor tissues were significantly lower compared ...
2021-01-25
Aging-US Issue 1 Volume 13 features "PAM (PIK3/AKT/mTOR) signaling in glia: potential contributions to brain tumors in aging" which reported that despite a growing proportion of aged individuals at risk for developing cancer in the brain, the prognosis for these conditions remains abnormally poor due to limited knowledge of underlying mechanisms and minimal treatment options.
While cancer metabolism in other organs is commonly associated with upregulated glycolysis and hyperactivation of PIK3/AKT/mTOR pathways, the unique bioenergetic demands of the central nervous system ...
LAST 30 PRESS RELEASES:
[Press-News.org] Titanium oxide nanotubes facilitate low-cost laser-assisted photoporation
