(Press-News.org) Tsukuba, Japan—In eukaryotes, genomic DNA, which is a very long double helix containing all the genetic information, wraps around a globular protein called a histone and folds it many times before being contained within the nucleus. Various post-translational modifications (for example, addition of chemical groups) occur on histones. Among them, the methylation of residues of lysine, which is one of the amino acids that make up histones, regulates the folding of genomic DNA and acts as a switch to turn gene transcription on and off.
The research group has discovered the methylation of histidine residues as a new post-translational modification of histones using a unique method to distinguish precisely the presence and mode of methylation of proteins. Histones form an octamer, which contains two copies each of the four core histone proteins H2A, H2B, H3, and H4. Among these, histidine methylation was found to occur at the 82nd histidine of histone H2A and at the 39th histidine of histone H3. Additionally, an examination of all the methylation states of histone H3 showed that most methylation modifications were concentrated on lysine residues, suggesting that methylation of histidine residues in histones occurs only in few histones in a specific gene region.
Histones contain many lysine residues, which undergo various post-translational modifications such as methylation and acetylation. The combination pattern is called the histone code, which is regarded as a code that directs transcriptional regulation. The discovery of the methylation of histidine residues is expected to be a new step toward deciphering the histone code.
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This work was supported by JST SPRING, Grant Number JPMJSP2124 (to T. H.), JSPS KAKENHI Grant Numbers 20H02947 (to H. D.), 17H01519 (to A. F.) and 23H00321 (to A. F.), and a grant from AMED-CREST Grant Number JP21gm1410010 (to A. F.).
Original Paper
Title of original paper:
Histidine Nτ-methylation identified as a new post-translational modification in histone H2A at His-82 and H3 at His-39
Journal:
The Jornal of Biological Chemistry
DOI:
10.1016/j.jbc.2023.105131
Correspondence
Assistant Professor DAITOKU, Hiroaki
Professor FUKAMIZU, Akiyoshi
Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA)
Related Link
Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA)
END
Discovery of histidine methylation as a new post-translational modification of histone proteins
2023-09-22
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