(Press-News.org)
“Overall, our study provides novel insight into how reproductive aging impacts the murine ovarian proteome and ECM.”
BUFFALO, NY- October 31, 2023 – A new priority research paper was published on the cover of Aging (listed by MEDLINE/PubMed as "Aging (Albany NY)" and "Aging-US" by Web of Science) Volume 15, Issue 20, entitled, “Proteomic quantification of native and ECM-enriched mouse ovaries reveals an age-dependent fibro-inflammatory signature.”
The ovarian microenvironment becomes fibrotic and stiff with age, in part due to increased collagen and decreased hyaluronan. However, the extracellular matrix (ECM) is a complex network of hundreds of proteins, glycoproteins, and glycans which are highly tissue specific and undergo pronounced changes with age. In this new study, researchers Shweta S. Dipali, Christina D. King, Jacob P. Rose, Joanna E. Burdette, Judith Campisi, Birgit Schilling, and Francesca E. Duncan from Northwestern University’s Feinberg School of Medicine, the Buck Institute for Research on Aging and the University of Illinois at Chicago used label-free quantitative proteomic methods to define comprehensive, age-dependent changes in the murine ovarian proteome and ECM in an unbiased manner.
“To obtain an unbiased and comprehensive profile of age-associated alterations to the murine ovarian proteome and ECM, we used a label-free quantitative proteomic methodology.”
The researchers validated conditions to enrich for the ECM prior to proteomic analysis. Following analysis by data-independent acquisition (DIA) and quantitative data processing, they observed that both native and ECM-enriched ovaries clustered separately based on age, indicating distinct age-dependent proteomic signatures. The team identified a total of 4,721 proteins from both native and ECM-enriched ovaries, of which 383 proteins were significantly altered with advanced age, including 58 ECM proteins.
Several ECM proteins upregulated with age have been associated with fibrosis in other organs, but to date their roles in ovarian fibrosis are unknown. Pathways regulating DNA metabolism and translation were downregulated with age, whereas pathways involved in ECM remodeling and immune response were upregulated. Interestingly, immune-related pathways were upregulated with age even in ECM-enriched ovaries, suggesting a novel interplay between the ECM and the immune system.
Moreover, the researchers identified putative markers of unique immune cell populations present in the ovary with age. These findings provide evidence from a proteomic perspective that the aging ovary provides a fibroinflammatory milieu, and their study suggests target proteins which may drive these age-associated phenotypes for future investigation.
“To our knowledge, this is the first study to utilize unbiased proteomic approaches to investigate the effect of reproductive aging on the murine ovarian proteome and matrisome.”
Read the full study: DOI: https://doi.org/10.18632/aging.205190
Corresponding Authors: Francesca E. Duncan, Birgit Schilling - f-duncan@northwestern.edu, bschilling@buckinstitute.org
Keywords: reproductive aging, ovary, proteomics, extracellular matrix, data-independent acquisition
Sign up for free Altmetric alerts about this article: https://aging.altmetric.com/details/email_updates?id=10.18632%2Faging.205190
About Aging:
Launched in 2009, Aging (Aging-US) publishes papers of general interest and biological significance in all fields of aging research and age-related diseases, including cancer—and now, with a special focus on COVID-19 vulnerability as an age-dependent syndrome. Topics in Aging go beyond traditional gerontology, including, but not limited to, cellular and molecular biology, human age-related diseases, pathology in model organisms, signal transduction pathways (e.g., p53, sirtuins, and PI-3K/AKT/mTOR, among others), and approaches to modulating these signaling pathways.
Please visit our website at www.Aging-US.com and connect with us:
SoundCloud
Facebook
X, formerly known as Twitter
Instagram
YouTube
LabTube
LinkedIn
Reddit
Pinterest
Click here to subscribe to Aging publication updates.
For media inquiries, please contact media@impactjournals.com.
Aging (Aging-US) Journal Office
6666 E. Quaker Str., Suite 1B
Orchard Park, NY 14127
Phone: 1-800-922-0957, option 1
###
END
Physicists William Raphael “Raph” Hix of the Department of Energy’s Oak Ridge National Laboratory and John Lajoie, who will join ORNL on Nov. 6 from Iowa State University, have been elected Fellows of the American Physical Society.
The society works to advance and spread physics knowledge via research journals, scientific meetings, education, outreach, advocacy and international activities. It represents more than 50,000 members, including physicists in government, academia and industry worldwide.
Hix, leader of the Theoretical and Computational ...
CHICAGO—October 31, 2023—Researchers at Illinois Institute of Technology (Illinois Tech) have been recognized by Peoples Gas with the Innovation Strategies and Technologies Award for their Battery-Operated Radiator Control (BORC) system, a groundbreaking solution to optimize the efficiency of manually operated radiators.
The researchers, Assistant Professor of Architectural Engineering Mohammad Heidarinejad and Arthur W. Hill Endowed Chair in Sustainability Brent Stephens, had the insight that traditional steam radiators are ...
Recent preclinical results indicate novel next-generation vaccine candidates developed at Cleveland Clinic protect against multiple strains of influenza and last longer than vaccines currently in use.
The vaccines are part of Cleveland Clinic's global vaccine research program, led by Ted Ross, PhD, Global Director of Vaccine Development at Cleveland Clinic. Published in Scientific Reports, the study credits the preclinical success of the influenza B vaccines to novel technology called Computationally Optimized Broadly Reactive ...
ITHACA, N.Y. – Book publishers cried foul – in the form of numerous legal challenges – nearly two decades ago when the Google Books project digitized and freely distributed more than 25 million works.
The publishers argued that free digital distribution undermines the market for physical books, but new research from Cornell University’s Imke Reimers and a collaborator reveals that the opposite – increased demand for physical books, through online discovery – could be true.
Reimers, an associate professor in the Charles H. Dyson School of Applied Economics and Management, and Abhishek Nagaraj, assistant professor ...
The U.S. Department of Energy's National Renewable Energy Laboratory (NREL) is preparing to unveil a database containing the results of exposure experiments on solar reflectors conducted over more than four decades. The publicly available Solar Mirror Materials Database (SMMD) will contain information from thousands of solar mirror samples from more than a hundred suppliers that have been subjected to outdoor tests and laboratory environments.
Typically used for concentrating solar-thermal power, these mirrors were installed and tested in Phoenix, ...
Increasing human input when AI is used for public services boosts acceptance of the technology, a new study shows.
The research shows citizens are not only concerned about AI fairness but also about potential human biases. They are in favour of AI being used in cases when administrative discretion is perceived as too large.
Researchers found citizens' knowledge about AI does not alter their acceptance of the technology. More accurate systems and lower cost systems also increased their acceptance. Cost and accuracy of technology ...
Obtaining high-resolution images in the world of microscopy has long been a challenge. Deconvolution, a method to enhance image clarity, often amplifies noise between the sample and the image. Researchers at Boston University recently developed a novel deblurring algorithm that avoids these issues, improving the resolution of images with photon intensity conservation and local linearity.
As reported in the Gold Open Access journal Advanced Photonics, the innovative deblurring algorithm is adaptable to various fluorescence microscopes, requiring minimal assumptions about the emission point spread function (PSF). It works on both a sequence of raw images and even a single image, enabling ...
New research led by a Florida State University professor shows that potential adaptive responses by sea turtles, such as shifting the timing of when they nest, may not be enough to counteract the projected impacts from climate change on hatchling production.
Warmer temperatures cause lower hatchling success and a greater percentage of female turtles, both of which can disrupt the viability of a species. Sand temperatures at sea turtle nesting sites globally are projected to increase by about 0.6 degrees Celsius to ...
Suicide and mental health distress disproportionately affect veterans in the United States. According to a report from the Substance Abuse and Mental Health Services Administration, more than 5 million veterans suffered from these adverse behavioral health issues in 2020. That same year, after adjusting for age and sex differences in the population, the U.S. Department of Veterans Affairs (VA) estimated that veterans were 57.3 percent more likely to commit suicide than non-veterans.
While the Veterans ...
The First ‘Birder’s Guide’ to Meteor Showers
Peter Jenniskens new comprehensive guide describes over 500 meteor showers that appear in our night skies.
October 31, 2023, Mountain View, CA -- The SETI Institute is proud to announce a new book by SETI Institute meteor astronomer Dr. Peter Jenniskens. Called “Atlas of Earth’s Meteor Showers,” this comprehensive guide describes over 500 meteor showers that appear in our night skies and adds a wealth of detail to the tapestry of our solar system.
“Just as in a birder’s guide, the book describes the outward appearance, ...
