(Press-News.org) MAYWOOD, Ill. -- A scientific method paper and video by Loyola researchers has gone viral. The video demonstrates a laboratory technique used to study some aspects of mitochondrial dysfunctions in Alzheimer's disease and many other disorders.
It has been accessed by more than 14,000 scientists around the world since it was published in the Journal of Visualized Experiments, a peer-reviewed, PubMed-indexed journal that publishes biological and other scientific research in a video format.
Senior author is Joanna C. Bakowska, DVM, PhD, an assistant professor in the Department of Pharmacology of Loyola University Chicago Stritch School of Medicine. First author is Dinesh Joshi, PhD.
The 10-minute video is accompanied by an article. In it, the researchers detail how to measure the mitochondrial membrane potential -- the electrical potential across the inner mitochondrial membrane.
Mitochondria are the power producers of the cell. They convert energy into forms that the cell can use. The mitochondria's production of energy is correlated with the membrane potential. An abnormal membrane potential has been demonstrated in several neurological disorders, including Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis, also known as Lou Gehrig's disease. It also is found in many other conditions, including tumors and alcoholic liver disease.
Bakowska and Joshi demonstrated a technique that uses a specific fluorescent dye and live cell imaging to determine the mitochondrial membrane potential. The video provides a step-by-step guide on how to conduct the technique.
"Scientists will be able to do this technique on their own, just after watching the video," Bakowska said.
Their work was supported by the National Institutes of Health.
The Journal of Visualized Experiments (JoVE) has opened a new frontier in scientific publication. It enables scientists to publish experiments in all dimensions, overcoming the limitations of traditional print journals.
This article in JoVE is free with open access.
"We wanted the article and video to be as widely disseminated as possible," Bakowska said.
INFORMATION: END
A consortium of federal agencies and nongovernmental organizations has published a report proposing a set of common measures and data-collection methods for use in asthma clinical research. Asthma Outcomes in Clinical Research: Report of the Asthma Outcomes Workshop, which appears as a supplement to the March 2012 issue of the Journal of Allergy and Clinical Immunology, resulted from a meeting organized by the National Institutes of Health, the Agency for Healthcare Research and Quality and the Merck Childhood Asthma Network, Inc., with additional support for the publication ...
As more organizations look for ways to manage the cost of employee healthcare, what better way to bring this message to your organization than to have your CEO enter a fitness challenge. Nancy Munro, CEO of KnowledgeShift was willing to take on this challenge offered to members of the Illinois Technology Association starting in November of 2011. The contest was sponsored by InerTrain, an online fitness company.
Contestants had to evaluate their fit age before and after the contest. Each contestant worked with one of InerTrain's online virtual trainers with personalized ...
Scientists from PRBO Conservation Science and the Department of Fish and Game have completed an innovative study on the effects of climate change on bird species of greatest concern. This first-of-its-kind study prioritizes which species are most at risk and will help guide conservation measures in California. The study was published this week in the journal PLoS ONE.
"What's most exciting about the study is that our unique approach is one that other scientists and resource managers can duplicate to help them conserve wildlife in the face of climate change," said PRBO ...
A University of Alberta research team has discovered that technology commonly used to decontaminate food industry equipment can also rid meat processing plants of lethal microbial material responsible for the human version of the ailment Mad Cow disease.
U of A microbiology professors Mike Belosevic and Norm Neumann and engineering professor Mohamed Gamal El-Din demonstrated that infectious proteins found in the brain matter of cattle can be eradicated from water treated with ozone.
The discovery could have applications in decontaminating wastewater in settings such ...
Athens, Ga. – In an emergency, minutes matter. With this knowledge, University of Georgia researchers developed a new method for determining where emergency vehicle stations should be located. The results of their work could improve ambulance response time for the 200 million Americans who dial 911 each year, according to the Federal Communications Commission.
"If we can meet this critical time window [of 8 minutes], we can maximize benefits," said Ping Yin, a UGA graduate student studying geography who co-authored the paper.
The model uses geographical information ...
A polymer is a mesh of chains, which slowly break over time due to the pressure from ordinary wear and tear. When a polymer is squeezed, the pressure breaks chemical bonds and produces free radicals: ions with unpaired electrons, full of untapped energy. These molecules are responsible for aging, DNA damage and cancer in the human body.
In a new study, Northwestern University scientists turned to squeezed polymers and free radicals in a search for new energy sources. They found incredible promise but also some real problems. Their report is published by the journal Angewandte ...
Unionville Invisalign specialist Dr. Arun Rajasekaran and his staff are proud of the fact that clients routinely refer friends and family to Liberty Orthodontic Centre.
Patient referrals are a vote of confidence in the practice, and Rajasekaran and his team enjoy seeing new patients who they can help to achieve an ideal smile, says Marketing Coordinator Laura Lindsay.
Whether patients are searching for more general orthodontics and braces or a specific teeth straightening procedure such as Invisalign, referrals are an indicator of how well the office serves its patients, ...
WASHINGTON -- The exhaust fumes from gasoline vehicles contribute more to the production
of a specific type of air pollution-secondary organic aerosols (SOA)-than those from diesel
vehicles, according to a new study by scientists from the Cooperative Institute for Research in
Environmental Sciences (CIRES), NOAA's Earth System Research Laboratory (ESRL) and
other colleagues.
"The surprising result we found was that it wasn't diesel engines that were contributing the
most to the organic aerosols in LA," said CIRES research scientist Roya Bahreini, who led
the ...
Our senses aren't just delivering a strict view of what's going on in the world; they're affected by what's going on in our heads. A new study finds that hungry people see food-related words more clearly than people who've just eaten. The study, published in Psychological Science, a journal of the Association for Psychological Science, finds that this change in vision happens at the earliest, perceptual stages, before higher parts of the brain have a chance to change the messages coming from the eyes.
Psychologists have known for decades that what's going on inside our ...
San Diego homebuyers seeking new, detached homes in uniquely desirable Tierrasanta can join the VIP interest list for Copperleaf, where a spring preview is planned ahead of the model grand opening. The preview period will feature guided "dusty shoe" tours of the model homes under construction, and additional opportunities to get a head start on purchasing a new Copperleaf home.
For the latest information and updates, you can join the growing interest list at www.copperleafsd.com or you can call 858-535-9033. Buyers who pre-qualify on the website will automatically ...
