Going viral to kill zits

(Press-News.org) Watch out, acne. Doctors soon may have a new weapon against zits: a harmless virus living on our skin that naturally seeks out and kills the bacteria that cause pimples.

The Sept. 25 online edition of the American Society for Microbiology's mBio publishes the findings by scientists at UCLA and the University of Pittsburgh.

"Acne affects millions of people, yet we have few treatments that are both safe and effective," said principal investigator Dr. Robert Modlin, chief of dermatology and professor of microbiology, immunology and molecular genetics at the David Geffen School of Medicine at UCLA. "Harnessing a virus that naturally preys on the bacteria that causes pimples could offer a promising new tool against the physical and emotional scars of severe acne."

The scientists looked at two little microbes that share a big name: Propionibacterium acnes, a bacterium thriving in our pores that can trigger acne; and P. acnes phages, a family of viruses that live on human skin. The viruses are harmless to humans, but programmed to infect and kill the aforementioned P. acnes bacteria.

When P. acnes bacteria aggravate the immune system, it causes the swollen, red bumps associated with acne. Most effective treatments work by reducing the number of P. acnes bacteria on the skin.

"We know that sex hormones, facial oil and the immune system play a role in causing acne, however, a lot of research implicates P. acnes as an important trigger," explained first author Laura Marinelli, a UCLA postdoctoral researcher in Modlin's laboratory. "Sometimes they set off an inflammatory response that contributes to the development of acne."

Using over-the-counter pore cleansing strips from the drugstore, the researchers lifted acne bacteria and the P. acnes viruses from the noses of both pimply and clear-skinned volunteers.

When the team sequenced the bacteriophages' genomes, they discovered that the viruses possess multiple features – such as small size, limited diversity and the broad ability to kill their hosts – that make them ideal candidates for the development of a new anti-acne therapy.

"Our findings provide valuable insights into acne and the bacterium that causes it," observed corresponding author Graham Hatfull, Eberly Family Professor of Biotechnology, professor of biological sciences at the University of Pittsburgh and a Howard Hughes Medical Institute researcher. "The lack of genetic diversity among the phages that attack the acne bacterium implies that viral-based strategies may help control this distressing skin disorder."

"Phages are programmed to target and kill specific bacteria, so P. acnes phages will attack only P. acnes bacteria, but not others like E. coli," added Marinelli. "This trait suggests that they offer strong potential for targeted therapeutic use."

Acne affects nearly 90 percent of Americans at some point in their lives, yet scientists know little about what causes the disorder and have made narrow progress in developing new strategies for treating it. Dermatologists' arsenal of anti-acne tools -- benzoyl peroxide, antibiotics and Accutane – hasn't expanded in decades.

"Antibiotics such as tetracycline are so widely used that many acne strains have developed resistance, and drugs like Accutane, while effective, can produce risky side effects, limiting their use," explained coauthor Dr. Jenny Kim, director of the UCLA Clinic for Acne, Rosacea and Aesthetics. "Acne can dramatically disfigure people and undermine their self-esteem, especially in teens. We can change patients' lives with treatment. It's time we identified a new way to safely treat the common disorder."

The research team plans to isolate the active protein from the P. acnes virus and test whether it is as effective as the whole virus in killing acne bacteria. If laboratory testing proves successful, the researchers will study the compound's safety and effectiveness in combating acne in people.

### The study was supported by grants from the National Institute of Arthritis and Musculoskeletal and Skin Diseases (R21AR060382, R01 AR053542 and F32AR060655) at the National Institutes of Health in Bethesda, Md.

Additional coauthors included Sorel Fitz-Gibbon, Megan Inkeles, Shawn Cokus, Matteo Pellegrini and Jeffrey F. Miller, all of UCLA; former UCLA researchers Clarmyra Hayes and Anya Loncaric, now of the California Institute of Technology and Solta Medical, respectively; and Charles Bowman, Daniel Russell and Deborah Jacobs-Sera of the University of Pittsburgh.

The Clinic for Acne, Rosacea and Aesthetics at the UCLA Division of Dermatology at the David Geffen School of Medicine offers comprehensive care for acne and rosacea, as well as the scarring and discoloration that can result from these conditions. The clinic's goal is to educate the public and help patients develop habits leading to healthy skin. Current research projects include studying the effect of Vitamin-D on immune response to acne, the effect of Omega-3 fatty acids on acne and its treatment, and the use of a mobile device application for acne management. To schedule an appointment, call (310) 825-6911.