Lateral flow testing should not be used as a green light for activities
Study suggests the screening tool is more effective at detecting higher quantities of viral RNA
2021-04-29
(Press-News.org) The United Kingdom government plans to implement mass scale population testing for SARS-CoV-2 infection using Lateral Flow Devices (LFDs), yet the devices' sensitivity is unknown. A study published in the open access journal PLOS Biology by Alan McNally at University of Birmingham, UK, and colleagues suggests while LFDs are highly effective in identifying SARS-CoV-2 in individuals with high quantities of viral RNA present on the test swab, they are inaccurate at diagnosing infections in individuals with lower viral loads.
LFDs are increasingly used to increase testing capacity and screen asymptomatic populations for SARS-CoV-2 infection in mass surveillance programs, yet there are few data indicating the performance of these devices. To determine the sensitivity of LFDs, researchers tested the nasopharyngeal swabs of 8,000 university students using the devices. Next, they validated all positive samples and randomly selected 720 negative samples out of 7,187 negatives for validation.
Using reverse transcription PCR tests, the authors estimated the true COVID-19 prevalence rate and compared it to LFD test results. The researchers were able to determine the LFDs' accuracy rate for different levels of virus, finding that while the test was able to detect infections in samples with higher levels of viral RNA at a 100% accuracy rate, the test's performance dropped significantly in samples with lower viral loads.
The results suggest that for individuals in the very early or very late stages of infection, LFDs are likely to produce a false negative result, pointing to the need for frequent testing. A limitation of the study was that emergent variants and their potential effects on LFDs' sensitivity with asymptomatic cases were not considered within the scope of the study. Independent research suggests that both the UK and South African variants can be reliably detected, but further studies are needed to determine whether the LFDs can detect infections at the same viral loads as with the SARS-CoV-2 strain examined in the current research.
According to the authors, "When used regularly, LFDs can be a highly effective tool in reducing overall community burden. We would strongly recommend that Lateral Flow Device testing is used to screen people at a very regular frequency, and that a negative result should not be used to determine that someone is free from SARS-CoV-2 infection. As such LFDs should not be used as a test-to-do"
INFORMATION:
Research article
Peer reviewed; Experimental study; Humans
In your coverage please use these URLs to provide access to the freely available articles in PLOS Biology: http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3001216
Citation: Ferguson J, Dunn S, Best A, Mirza J, Percival B, Mayhew M, et al. (2021) Validation testing to determine the sensitivity of lateral flow testing for asymptomatic SARS-CoV-2 detection in low prevalence settings: Testing frequency and public health messaging is key. PLoS Biol 19(4): e3001216. https://doi.org/10.1371/journal.pbio.3001216
Funding: The PCR testing in this manuscript is funded by the UK Department for Health and Social Care (DHSC) as part of pillar 2 testing, in an award made directly to the University of Birmingham. The provision of LFD tests is funded by DHSC as part of a national student testing program, and funded directly to the University of Birmingham. DHSC have had no role in in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing Interests: The authors have declared that no competing interests exist.
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[Press-News.org] Lateral flow testing should not be used as a green light for activities
Study suggests the screening tool is more effective at detecting higher quantities of viral RNA
