(Press-News.org) Tsukuba, Japan—Proteins are subject to post-translational chemical modifications that result in functional diversity. Methylation is one such modification that is generally believed to occur on lysine and arginine residues. Recently, this modification has been shown to occur on histidine residues as well. Furthermore, it has been suggested that histidine methylation occurs in a wide range of proteins. However, many details remain unknown: for example, which are the proteins that are methylated and modified and which are the histidine residues in cells, tissues, or organs where such modifications occur are not known.
Using mouse skeletal muscle and brain tissue and using a combination of methods based on biochemical protein fractionation and analytical chemistry, the research group identified γ-enolase, a subunit of the glycolytic enzyme enolase, as a new substrate for the methylation of histidine residues. Among the three amino acid sequences of enolase (i.e., α, β, and γ), the 190th histidine residue of γ-enolase, which is specifically expressed in the central nervous system was found to be methylated.
A conformational prediction study suggested that the histidine residue that undergoes methylation in γ-enolase may be important for intermolecular hydrogen bonding during subunit dimer formation. It was found that the disruption of hydrogen bonding at this site of γ-enolase by substituting histidine with another residue resulted in the suppression of the subunit dimerization and enolase activity. None of the three histidine methyltransferases previously identified in mammals catalyzed the methylation reaction of γ-enolase. From these results, the newly identified γ-enolase is expected to be a unique research tool for exploring new histidine methyltransferases.
###
This work was supported by the Japan Society for the Promotion of Science Grant-in-aid for Scientific Research (A) (23H00321 to A.F.), Grant-in-aid for Scientific Research (B) (20H029947 to H.D.) and a grant from Japan Agency for Medical Research and Development, AMED-CREST (JPgm1410010 to A.F.).
Original Paper
Title of original paper:
γ-enolase (ENO2) is methylated at the Nτ position of His-190 among enolase isozymes
Journal:
The Journal of Biochemistry
DOI:
10.1093/jb/mvad042
Correspondence
Assistant Professor KAKO, Koichiro
Institute of Life and Environmental Sciences, University of Tsukuba
Professor FUKAMIZU, Akiyoshi
Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA)
Related Link
Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA)
Institute of Life and Environmental Sciences
END
New post-translational modification of the glycolytic enzyme enolase
2023-09-29
ELSE PRESS RELEASES FROM THIS DATE:
New frontier in biomedical engineering: Protein coacervates engineered into adhesive for unprecedented skin repair speed
2023-09-29
A team of researchers from China has made a significant breakthrough in biomedical engineering by developing a novel adhesive that promises to revolutionize wound management and tissue repair. The research, published in Engineering, unveils a biocomposite adhesive that exhibits robust adhesion and real-time skin healing properties.
Adhesives have long been recognized as a valuable tool in biomedical engineering. However, current adhesive systems face challenges in achieving strong and durable adhesion, limiting their effectiveness in wound healing. ...
New study unveils insights into ethylene copolymerization with linear and end-cyclized olefins using a metallocene catalyst
2023-09-29
A research team led by Changjiang Wu at SINOPEC (Beijing) Research Institute of Chemical Industry Co., Ltd. in China has made important progress in understanding the polymerization behavior and thermal properties of copolymers formed through ethylene copolymerization with linear and end-cyclized olefins. The findings, published in the journal Engineering, shed light on the potential of utilizing different comonomers in olefin solution polymerization to obtain high-performance polyolefin materials.
Polyolefin elastomers (POEs) are widely used in various industries due to their exceptional properties. However, the high cost ...
Study identifies new pathway to suppressing autoimmunity
2023-09-29
Researchers at Stanford University School of Medicine, Yale University School of Medicine, and the Hospital for Special Surgery Research Institute have uncovered new details about how the immune system prevents the production of antibodies that can recognize and damage the body’s own, healthy tissues. The study, to be published September 29 in the Journal of Experimental Medicine (JEM), also reveals how this process is impaired in autoimmune disorders such as systemic sclerosis and systemic lupus ...
Diabetes may accelerate blood cancer growth, yet survival outcomes differ by race
2023-09-29
(WASHINGTON, Sept. 29, 2023) – Patients with multiple myeloma, a blood cancer of plasma cells in the bone marrow, who also have diabetes have a reduced overall survival when compared to those without diabetes. In a subgroup analysis, this difference in survival due to diabetes was seen in white patients but not in Black patients, according to a study published today in Blood Advances.
According to the Centers for Disease Control and Prevention, diabetes affects 13% of the U.S. population, and this prevalence is growing rapidly. Multiple myeloma is the second most common blood cancer in the U.S. and disproportionately ...
Groundbreaking mathematical proof: new insights into typhoon dynamics unveiled
2023-09-29
In a remarkable breakthrough in the field of Mathematical Science, Professor Kyudong Choi from the Department of Mathematical Sciences at UNIST has provided an irrefutable proof that certain spherical vortices exist in a stable state. This groundbreaking discovery holds significant implications for predicting weather anomalies and advancing weather prediction technologies.
A vortex is a rotating region of fluid, such as air or water, characterized by intense rotation. Common examples include typhoons and tornadoes frequently observed in news reports. Professor Choi’s mathematical proof establishes the stability of specific ...
Teams invent a new metallization method of modified tannic acid photoresist patterning
2023-09-29
The micro/nano metal pattern formation is a key step in the assembly of various devices. However, ex situ approaches of metal patterning limited their industrial applications due to the poor stability and dispersion of metal nanoparticles. The in situ electroless deposition after lithography patterning may be a better choice for avoiding the growth and aggregation of metal particles in the polymers. Tannic acid is rich in natural products, having an adjacent tri-hydroxyl structure, which can realize the in situ reduction of metal ions on the photoresist pattern. A team of scientists ...
MoMFs could be central to liver regeneration
2023-09-29
The liver is a vital organ that plays a role in many essential functions, including digestion, detoxification, and metabolism. When the liver is damaged, it has the remarkable ability to regenerate itself. However, the process of liver regeneration is not fully understood.
A critical aspect of liver regeneration is removing dead tissue and necrotic lesions. In a recent study published in the Journal of Clinical Investigation, Feng et al. showed that monocyte-derived macrophages (MoMFs) play a crucial role in this process. MoMFs are a type of white blood cell that is recruited to the liver in response to injury. Once in the liver, MoMFs engulf dead cells ...
A lethal parasite’s secret weapon: Infecting non-immune cells
2023-09-29
COLUMBUS, Ohio – The organisms that cause visceral leishmaniasis, a potentially deadly version of the parasitic disease that most often affects the skin to cause disfiguring disease, appear to have a secret weapon, new research suggests: They can infect non-immune cells and persist in those uncommon environments.
Researchers found the Leishmania donovani parasites in blood-related stem cells in the bone marrow of chronically infected mice – precursor cells that can regenerate all types of cells in the blood-forming system. The finding may help explain why some people who develop visceral leishmaniasis, which is ...
Ball milling provides high pressure benefits to battery materials
2023-09-29
Cheaper, more efficient lithium-ion batteries could be produced by harnessing previously overlooked high pressures generated during the manufacturing process.
Scientists at the University of Birmingham have discovered that routine ball milling can cause high pressure effects on battery materials in just a matter of minutes, providing a vital additional variable in the process of synthesizing battery materials.
The research (part of the Faraday Institution funded CATMAT project), led by Dr Laura Driscoll, Dr Elizabeth Driscoll and Professor Peter Slater at the University of Birmingham is published in RSC Energy Environmental Science.
The use ...
Congenital Heart Surgeons' Society (CHSS) and fourteen professional organizations announce recommendations for performing pediatric heart surgery in US
2023-09-29
September 29, 2023, Cincinnati, OH — A set of recommendations to address the known variation in outcomes at US congenital heart surgery centers has been endorsed by 15 collaborating societies led by the Congenital Heart Surgeons' Society (CHSS). The guidelines will appear in “Recommendations for Centers Performing Pediatric Heart Surgery in the United States," to be co-published in the World Journal for Pediatric and Congenital Heart Surgery, Annals of Thoracic Surgery, and Journal of Thoracic and Cardiovascular Surgery.
“In ...