“Wearable” devices for cells

(Press-News.org) CAMBRDIGE, MA – Wearable devices like smartwatches and fitness trackers interact with parts of our bodies to measure and learn from internal processes, such as our heart rate or sleep stages.

Now, MIT researchers have developed wearable devices that may be able to perform similar functions for individual cells inside the body.

These battery-free, subcellular-sized devices, made of a soft polymer, are designed to gently wrap around different parts of neurons, such as axons and dendrites, without damaging the cells, upon wireless actuation with light. By snugly wrapping neuronal processes, they could be used to measure or modulate a neuron’s electrical and metabolic activity at a subcellular level.

Because these devices are wireless and free-floating, the researchers envision that thousands of tiny devices could someday be injected and then actuated noninvasively using light. Researchers would precisely control how the wearables gently wrap around cells, by manipulating the dose of light shined from outside the body, which would penetrate the tissue and actuate the devices.

By enfolding axons that transmit electrical impulses between neurons and to other parts of the body, these wearables could help restore some neuronal degradation that occurs in diseases like multiple sclerosis. In the long run, the devices could be integrated with other materials to create tiny circuits that could measure and modulate individual cells.

“The concept and platform technology we introduce here is like a founding stone that brings about immense possibilities for future research,” says Deblina Sarkar, the AT&T Career Development Assistant Professor in the MIT Media Lab and Center for Neurobiological Engineering, head of the Nano-Cybernetic Biotrek Lab, and the senior author of a paper on this technique.

Sarkar is joined on the paper by lead author Marta J. I. Airaghi Leccardi, a former MIT postdoc who is now a Novartis Innovation Fellow; Benoît X. E. Desbiolles, an MIT postdoc; Anna Y. Haddad ’23, who was an MIT undergraduate researcher during the work; and MIT graduate students Baju C. Joy and Chen Song. The research appears today in Nature Communications Chemistry.

 

Snugly wrapping cells

Brain cells have complex shapes, which makes it exceedingly difficult to create a bioelectronic implant that can tightly conform to neurons or neuronal processes. For instance, axons are slender, tail-like structures that attach to the cell body of neurons, and their length and curvature vary widely.

At the same time, axons and other cellular components are fragile, so any device that interfaces with them must be soft enough to make good contact without harming them.

To overcome these challenges, the MIT researchers developed thin-film devices from a soft polymer called azobenzene, that don’t damage cells they enfold.

Due to a material transformation, thin sheets of azobenzene will roll when exposed to light, enabling them to wrap around cells. Researchers can precisely control the direction and diameter of the rolling by varying the intensity and polarization of the light, as well as the shape of the devices.

The thin films can form tiny microtubes with diameters that are less than a micrometer. This enables them to gently, but snugly, wrap around highly curved axons and dendrites.

“It is possible to very finely control the diameter of the rolling. You can stop if when you reach a particular dimension you want by tuning the light energy accordingly,” Sarkar explains.

The researchers experimented with several fabrication techniques to find a process that was scalable and wouldn’t require the use of a semiconductor clean room.

 

Making microscopic wearables

They begin by depositing a drop of azobenzene onto a sacrificial layer composed of a water-soluble material. Then the researchers press a stamp onto the drop of polymer to mold thousands of tiny devices on top of the sacrificial layer. The stamping technique enables them to create complex structures, from rectangles to flower shapes.

A baking step ensures all solvents are evaporated and then they use etching to scrape away any material that remains between individual devices. Finally, they dissolve the sacrificial layer in water, leaving thousands of microscopic devices freely floating in the liquid.

Once they have a solution with free-floating devices, they wirelessly actuated the devices with light to induce the devices to roll. They found that free-floating structures can maintain their shapes for days after illumination stops.

The researchers conducted a series of experiments to ensure the entire method is biocompatible.

After perfecting the use of light to control rolling, they tested the devices on rat neurons and found they could tightly wrap around even highly curved axons and dendrites without causing damage.

“To have intimate interfaces with these cells, the devices must be soft and able to conform to these complex structures. That is the challenge we solved in this work. We were the first to show that azobenzene could even wrap around living cells,” she says.

Among the biggest challenges they faced was developing a scalable fabrication process that could be performed outside a clean room. They also iterated on the ideal thickness for the devices, since making them too thick causes cracking when they roll.

Because azobenzene is an insulator, one direct application is using the devices as synthetic myelin for axons that have been damaged. Myelin is an insulating layer that wraps axons and allows electrical impulses to travel efficiently between neurons.

In non-myelinating diseases like multiple sclerosis, neurons lose some insulating myelin sheets. There is no biological way of regenerating them. By acting as synthetic myelin, the wearables might help restore neuronal function in MS patients.

The researchers also demonstrated how the devices can be combined with optoelectrical materials that can stimulate cells. Moreover, atomically thin materials can be patterned on top of the devices, which can still roll to form microtubes without breaking. This opens up opportunities for integrating sensors and circuits in the devices.

In addition, because they make such a tight connection with cells, one could use very little energy to stimulate subcellular regions. This could enable a researcher or clinician to modulate electrical activity of neurons for treating brain diseases.

“It is exciting to demonstrate this symbiosis of an artificial device with a cell at an unprecedented resolution. We have shown that this technology is possible,” Sarkar says.

In addition to exploring these applications, the researchers want to try functionalizing the device surfaces with molecules that would enable them to target specific cell types or subcellular regions.

 

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The research was supported by the Swiss National Science Foundation and the U.S. National Institutes of Health Brain Initiative.

 

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