Screening approach enhances CRISPR genome-editing efficiency
(MEMPHIS, Tenn. – September 23, 2025) Natural systems such as CRISPR-associated transposons (CASTs) offer a targetable, one-step way to edit genomes. However, adapting them for biomedical applications has been challenging. To address this limitation, scientists at St. Jude Children’s Research Hospital designed a screening approach to measure efficiency and specificity for thousands of CAST variants accurately. This high-throughput approach allowed the researchers to rapidly optimize promising candidate ...