Spinach could lead to alternative energy more powerful than Popeye

(Press-News.org) WEST LAFAYETTE, Ind. — Spinach gave Popeye super strength, but it also holds the promise of a different power for a group of scientists: the ability to convert sunlight into a clean, efficient alternative fuel.

Purdue University physicists are part of an international group using spinach to study the proteins involved in photosynthesis, the process by which plants convert the sun's energy into carbohydrates used to power cellular processes.

"The proteins we study are part of the most efficient system ever built, capable of converting the energy from the sun into chemical energy with an unrivaled 60 percent efficiency," said Yulia Pushkar, a Purdue assistant professor of physics involved in the research. "Understanding this system is indispensible for alternative energy research aiming to create artificial photosynthesis."

During photosynthesis plants use solar energy to convert carbon dioxide and water into hydrogen-storing carbohydrates and oxygen. Artificial photosynthesis could allow for the conversion of solar energy into renewable, environmentally friendly hydrogen-based fuels.

In Pushkar's laboratory, students extract a protein complex called Photosystem II from spinach they buy at the supermarket. It is a complicated process performed over two days in a specially built room that keeps the spinach samples cold and shielded from light, she said.

Once the proteins have been carefully extracted, the team excites them with a laser and records changes in the electron configuration of their molecules.

"These proteins require light to work, so the laser acts as the sun in this experiment," Pushkar said. "Once the proteins start working, we use advanced techniques like electron paramagnetic resonance and X-ray spectroscopy to observe how the electronic structure of the molecules change over time as they perform their functions."

Photosystem II is involved in the photosynthetic mechanism that splits water molecules into oxygen, protons and electrons. During this process a portion of the protein complex, called the oxygen-evolving complex, cycles through five states in which four electrons are extracted from it, she said.

The international team recently revealed the structure of the first and third states at a resolution of 5 and 5.5 Angstroms, respectively, using a new technique called serial femtosecond crystallography. A paper detailing the results was published in Nature and is available online. In addition to Pushkar, Purdue postdoctoral researcher Lifen Yan and former Purdue graduate student Katherine Davis participated in the study and are paper co-authors.

Petra Fromme, professor of chemistry and biochemistry at Arizona State University, leads the international team.

"The trick is to use the world's most powerful X-ray laser, named LCLS, located at the Department of Energy's SLAC National Accelerator Laboratory," said Fromme in a statement. "Extremely fast femtosecond (one-quadrillionth of a second) laser pulses record snapshots of the PSII crystals before they explode in the X-ray beam, a principle called 'diffraction before destruction.'"

While X-ray crystallography reveals structural changes, it does not provide details of how the electronic configurations evolve over time, which is where the Purdue team's work came in. The Purdue team mimicked the conditions of the serial femtosecond crystallography experiment, but used electron paramagnetic resonance to reveal the electronic configurations of the molecules, Pushkar said.

"The electronic configurations are used to confirm what stage of the process Photosystem II is in at a given time," she said. "This information is kind of like a time stamp and without it the team wouldn't have been able to put the structural changes in context."

INFORMATION: The National Science Foundation and Department of Energy funded the Purdue team's work.

Writer: Elizabeth Gardner, 765-494-2081, ekgardner@purdue.edu

Source: Yulia Pushkar, 765-496-3279, ypushkar@purdue.edu

ASU news release:

https://asunews.asu.edu/20140709-water-splitting-photosynthesis

ABSTRACT

Serial time-resolved crystallography of photosystem II using a femtosecond X-ray laser

Names of researchers who wrote the article (format is "Abstract-AUTHORS") Christopher Kupitz, Shibom Basu, Ingo Grotjohann, Raimund Fromme, Nadia A. Zatsepin, Kimberly N. Rendek,
 Mark S. Hunter, Robert L. Shoeman, Thomas A. White, Dingjie Wang, Daniel James, Jay-How Yang, Danielle E. Cobb, Brenda Reeder, Raymond G. Sierra, Haiguang Liu, Anton Barty, Andrew L. Aquila, Daniel Deponte, Richard A. Kirian, Sadia Bari, Jesse J. Bergkamp, Kenneth R. Beyerlein, Michael J. Bogan, Carl Caleman, Tzu-Chiao Chao, 
Chelsie E. Conrad, Katherine M. Davis, Holger Fleckenstein, Lorenzo Galli, Stefan P. Hau-Riege, Stephan Kassemeyer, Hartawan Laksmono, Mengning Liang, Lukas Lomb, Stefano Marchesini, Andrew V. Martin, Marc Messerschmidt, Despina Milathianaki, Karol Nass, Alexandra Ros, Shatabdi Roy-Chowdhury, Kevin Schmidt, Marvin Seibert, 
Jan Steinbrener, Francesco Stellato, Lifen Yan, Chunhong Yoon, Thomas A. Moore, Ana L. Moore, Yulia Pushkar, 
Garth J. Williams, Se ́bastien Boutet, R. Bruce Doak, Uwe Weierstall, Matthias Frank, Henry N. Chapman, 
John C. H. Spence & Petra Fromme

Photosynthesis, a process catalysed by plants, algae and cyanobacteria converts sunlight to energy, thus sustaining all higher life on Earth. Two large membrane protein complexes, photosystem I and II (PSI andPSII), act in series to catalyse the light-driven reactions in photosynthesis. PSII catalyses the light-driven water splitting process, which maintains the Earth's oxygenic atmosphere. In this process, the oxygen-evolving complex (OEC) of PSII cycles through five states, S0 to S4, in which four electrons are sequentially extracted from the OEC in four light-driven charge separation events. Here we describe time resolved experiments on PSII nano/microcrystals from Thermosynechococcus elongates performed with the recently developed technique of serial femtosecond crystallography. Structures have been determined from PSII in the dark S1 state and after double laser excitation (putative S3 state) at 5 and 5.5. Å resolution, respectively. The results provide evidence that PSII undergoes significant conformational changes at the electron acceptor side and at the Mn4CaO5 core of the OEC. These include an elongation of the metal cluster, accompanied by changes in the protein environment, which could allow for binding of the second substrate water molecule between the more distant protruding Mn (referred to as the 'dangler' Mn) and the Mn3CaOx cubane in the S2 to S3 transition, as predicted by spectroscopic and computational studies. This work shows the great potential for time-resolved serial femtosecond crystallography for investigation of catalytic processes in biomolecules.