Study links genetic variants to higher 'bad' cholesterol and heart attack risk

(Press-News.org) PITTSBURGH, Oct. 30, 2025 – An international team led by a University of Pittsburgh School of Medicine scientist has created a first-of-its-kind resource to identify those with a genetic risk for elevated ‘bad’ cholesterol -- a major contributor to heart disease.

Published today in Science, this resource can assist clinicians in predicting patient risk for heart attacks and strokes, allowing time for prevention and early treatment.

Heart disease remains the leading cause of death in the United States, claiming nearly 700,000 lives each year. While diet and exercise play a role, the risk of heart disease is largely dictated by genetic predisposition to the accumulation of waxy plaque inside the arteries that supply blood to the heart. This predisposition arises from minor variations in the gene that encodes the receptor for a sticky molecule known as LDL, or low-density lipoprotein, commonly referred to as bad cholesterol.

“Even with normal LDL levels, a person might be at an elevated risk of a heart attack due to disease-causing variants in the LDL receptor,” said senior author Frederick Roth, Ph.D., professor and chair of computational and systems biology at Pitt. “By identifying damaging LDL receptor variants, clinicians can initiate preventive treatment early on and mitigate risks.”

In healthy blood vessels, LDL acts as a shuttle, transporting bits of ‘good’ cholesterol—an essential component of cell membranes that is also important for food digestion and the production of hormones and vitamins, including vitamin D. However, genetic mutations that lower the amount or efficiency of the LDL receptor can lead to harmfully high levels of LDL.

While modern gene sequencing technologies can read and decode a person’s entire genetic code from a small tissue sample within hours, interpreting such a vast amount of data is challenging, especially since the functional impact of most variations in the LDL receptor gene has previously been unknown.

In their new paper, Roth and team classified nearly 17,000 modifications of the LDL receptor gene along with the corresponding changes in the LDL receptor protein structure. The resulting table measures each protein variant based on its mechanism of action and its impact on LDL clearance efficiency, providing clinicians with potentially actionable insights into their patients’ risk of elevated LDL.

“New unclassified variants are seen all the time in the clinic, and we often don't have the evidence we need to inform patient care,” said study co-author Dan Roden, M.D., a clinician-scientist at Vanderbilt University Medical Center. “These variant impact scores have the potential to increase the number of diagnoses of familial high cholesterol for those with unclassified variants by a factor of ten.”

This cholesterol-specific effort is a part of a broader research community initiative co-founded by Roth to map the functional effects of genetic variants across inherited disorders, called the Atlas of Variant Effects Alliance, which now includes more than 500 scientists from 50 countries, all working to build similarly comprehensive maps that evaluate gene variants known to affect risk of many diseases on a large scale.

Similar to how identifying mutations in the BRCA1 breast cancer gene has saved many lives by predicting cancer risk early enough for prevention, the authors hope this resource will provide a powerful clinical diagnostic tool for heart disease.

The study also identified a subset of LDL receptor variants for which the ability to take up LDL was inhibited by high levels of very low-density lipoprotein (VLDL), a larger precursor of LDL. “The influence of VLDL on LDL uptake was an unexpected finding. We’re excited about investigating this further and understanding potential implications for human health,” said lead author Daniel Tabet, Ph.D., of the University of Toronto.

Key experimental work was led by co-author Atina Coté, Ph.D., at the Lunenfeld-Tanenbaum Research Institute, Sinai Health in Toronto. Other authors included Calum MacRae, M.D., Ph.D., a clinician-scientist at Brigham and Women’s Hospital who helped conceive the study, and Megan Lancaster, M.D. Ph.D., of VUMC, who helped relate the data to cardiac traits in a large human cohort.  

This research was conducted in the context of the multi-site CardioVar center supported by the National Heart, Lung, and Blood Institute (NHLBI) of the National Institutes of Health (NIH; grant HL164675); by the NIH National Human Genome Research Institute (NHGRI) though the Center of Excellence in Genomic Science Initiative (grant HG010461); and by the One Brave Idea Initiative (jointly funded by the American Heart Association, Verily Life Sciences LLC and Astra-Zeneca, Inc.); Canada Foundation for Innovation; Canadian Institutes of Health Research Foundation Grant; and The Canada Excellence Research Chairs Program.

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